Goat anti-Rabbit IgG Antibody
-
- Target See all IgG products
- IgG
-
Reactivity
- Rabbit
-
Host
- Goat
-
Clonality
- Polyclonal
-
Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)
- Supplier Product No.
- 611-1103
- Supplier
- Rockland
- Purpose
- Rabbit IgG Fc Antibody
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab')2.
- Characteristics
- Anti-Rabbit IgG (H&L) Antibody generated in goat detects reactivity to Rabbit IgG.
- Purification
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose.
- Sterility
- Sterile filtered
- Immunogen
-
Immunogen: Anti-Rabbit IgG was produced by repeated immunization with rabbit IgG f(c) fragment in goat
Immunogen Type: Native Protein
- Isotype
- IgG
-
-
- Application Notes
- Application Note: Antibody Anti-Rabbit IgG F(c) has been tested by ELISA, dot blot, and western blot and is suitable for immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 2.26 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- Expiry Date
- 12 months
-
- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
- Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
-