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Rabbit anti-Mouse IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP))

ELISA, IHC, WB Alkaline Phosphatase (AP)
Catalog No. ABIN287325

Quick Overview for Rabbit anti-Mouse IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) (ABIN287325)

Target

IgG

Conjugate

  • 665
  • 422
  • 422
  • 398
  • 274
  • 262
  • 235
  • 118
  • 105
  • 72
  • 67
  • 64
  • 61
  • 58
  • 58
  • 50
  • 44
  • 43
  • 40
  • 39
  • 39
  • 37
  • 35
  • 35
  • 32
  • 31
  • 31
  • 30
  • 29
  • 29
  • 29
  • 29
  • 29
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 27
  • 26
  • 26
  • 25
  • 21
  • 21
  • 19
  • 17
  • 17
  • 16
Alkaline Phosphatase (AP)

Application

  • 2119
  • 2050
  • 1885
  • 1873
  • 999
  • 920
  • 896
  • 520
  • 424
  • 234
  • 234
  • 149
  • 102
  • 98
  • 88
  • 84
  • 51
  • 33
  • 30
  • 28
  • 25
  • 20
  • 15
  • 11
  • 9
  • 8
  • 7
  • 5
  • 5
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
  • Binding Specificity

    • 2397
    • 517
    • 417
    • 146
    • 130
    • 128
    • 66
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Heavy & Light Chain

    Reactivity

    • 753
    • 703
    • 607
    • 468
    • 410
    • 244
    • 213
    • 176
    • 146
    • 141
    • 124
    • 110
    • 107
    • 101
    • 82
    • 62
    • 47
    • 28
    • 27
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse

    Host

    • 1773
    • 1583
    • 567
    • 282
    • 122
    • 112
    • 41
    • 32
    • 22
    • 21
    • 16
    • 11
    • 11
    Rabbit

    Specificity

    Yields a single arc in IEP against anti-Rabbit Serum, anti-Alkaline Phosphatase, Mouse Serum and Mouse IgG.

    Characteristics

    Rabbit anti Mouse IgG (H + L) secondary antibody (Alk Phos)
    Alternative Names: Rabbit anti Mouse Immunoglobulin G (H + L) secondary antibody (Alk Phos)

    Purification

    Affinity Chromatography

    Immunogen

    Rabbit anti-mouse IgG (H+L) (Alk Phos) was raised in rabbit using murine IgG whole molecule as the immunogen.
  • Application Notes

    ELISA: 1:2,000-1:10,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,500

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    0.05 M TRIS-HCL, pH 8.0, with 0.15 NaCl, 0.005 MgCl, 0.0005 ZnCl, 50 % Glycerol, 10 mg/mL BSA, and 0.01 % NaN3. Immunoglobulin and protease free.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Do not add Sodium azide.
    Dilute only prior to immediate use
    Each reagent is stable for the period shown on the bottle label if stored as directed.

    Storage

    4 °C
  • Target

    IgG

    Target Type

    Antibody
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