Mouse anti-Cow IgL Antibody

Catalog No. ABIN294144

Quick Overview for Mouse anti-Cow IgL Antibody (ABIN294144)

Target: IgL

Clonality: Monoclonal

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: IVA285-1

Isotype: IgG

Product Details

Reactivity: Cow

Host: Mouse

Specificity: Reacts with bovine IgG (light chains). IgG light chains (kappa and lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The antibody recognizes the light chain regardless it is free or attached to the heavy chain.

Purification: Protein A purified

Immunogen: Fraction of Bovine immunoglobulin.

Application Details

Application Notes: Approved: Flo, IHC, IHC-Fr, WB (1:50, 5 - 1 μg/mL)

Usage: Western Blotting: Recommended dilution - 0,5-1 μg/mL, overnight in 4 degrees C. Positive control and sample preparation: BS (bovine serum), dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min. Application notes:. Strongly reacts with

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide, approx,  pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details

Target: IgL

Alternative Name: Ig Light Chain