Mouse anti-Duck IgY Antibody

Catalog No. ABIN305009

Quick Overview for Mouse anti-Duck IgY Antibody (ABIN305009)

Target: IgY

Clonality: Monoclonal

Application: ELISA, Flow Cytometry (FACS), Western Blotting (WB)

Clone: 14A3

Isotype: IgG1

Product Details

Reactivity: Duck

Host: Mouse

Specificity: Recognizes the light chain of duck immunoglobulin, shown to be expressed strongly on bursal cells and weakly on splenocytes and peripheral lymphoid cells, but not expressed by thymocytes. Double-staining with clone 14A3 and duck CD8 alpha clone Du CD8-1, revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+ /14A3+ cells in duck bursa. The recognition of B cells by 14A3 was confirmed using a recently developed Flag-tagged chicken B cell marker, known as chBAFF-Flag (B cell activating factor). Recognizes IgA in Peking duck bile and can be used as a marker to identify B cells in both Peking and Muscovy duck and geese studies. Detects a band of approximately 25 kD in duck cell lysates in Western blotting.

No Cross-Reactivity: Chicken

Characteristics: Antigene is shown to be expressed strongly on bursal cells and weakly on splenocytes and peripheral lymphoid cells, but not expressed by thymocytes. Double-staining with clone 14A3 and duck CD8 alpha clone Du CD8-1, revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+ /14A3+ cells in duck bursa. The recognition of B cells by 14A3 was confirmed using a recently developed Flag-tagged chicken B cell marker, known as chBAFF-Flag (B cell activating factor). Recognizes IgA in Peking duck bile and can be used as a marker to identify B cells in both Peking and Muscovy duck and geese studies. Detects a band of approximately 25 kDa in duck cell lysates in Western blotting.

Purification: Affinity purified

Immunogen: Purified Peking duck yolk IgY.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA, Flo, WB

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request.

Comment:

Target Species of Antibody: Duck

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.

Storage: -20 °C

Storage Comment: Short term: -20°C
Long term: -20°C.

Target Details

Target: IgY

Target Type: Antibody

Molecular Weight: 25 kDa