Mouse anti-Duck IgY (N-Term) Antibody

Catalog No. ABIN305010

Quick Overview for Mouse anti-Duck IgY (N-Term) Antibody (ABIN305010)

Target: IgY

Clonality: Monoclonal

Application: ELISA, Flow Cytometry (FACS), Western Blotting (WB)

Clone: 16C7

Isotype: IgG1

Product Details

Binding Specificity: N-Term

Reactivity: Duck

Host: Mouse

Specificity: Recognizes both the IgY and IgY(-Fc) Peking duck heavy chain molecules. The duck IgY molecule, consisting of two heavy (H) and two light (L) chains, has been categorized as the 7.8S full-length IgY form and the 5.7S truncated IgY(-Fc) form. Studies have shown that the IgY(-Fc) molecule possesses the H chain Cu1 and Cu2 constant domains, but is lacking the Cu3 and Cu4 constant region terminal domains, all four of which are present in the full-length IgY molecule. Thus the structure of the IgY(-Fc) molecule resembles that of a F(ab')2 fragment of IgY. Detects IgY heavy chain in Mallard ducks, goose and swan. Detects bands of approximately 118kD and 178-200kD in Peking duck cell lysates under non-reducing conditions in Western blotting.

No Cross-Reactivity: Chicken

Characteristics: Detects IgY heavy chain in Mallard ducks, goose and swan. Detects bands of approximately 118 kDa and 178-200 kDa in Peking duck cell lysates under non-reducing conditions in Western blotting.

Purification: Affinity purified

Immunogen: Purified Peking duck yolk IgY.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA, Flo, WB

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL.

Comment:

Target Species of Antibody: Duck

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Target Details

Target: IgY

Target Type: Antibody

Molecular Weight: 118 kDa and 178-200 kDa