Rabbit anti-Mouse IgG2b Antibody (FITC)

Catalog No. ABIN339391

Quick Overview for Rabbit anti-Mouse IgG2b Antibody (FITC) (ABIN339391)

Target: IgG2b

Clonality: Polyclonal

Conjugate: FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Rabbit

Specificity: Recognizes mouse IgG2b, g2b chain. Assay by IEP resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum and mouse serum. Specificity was confirmed by ELISA at less than 1 % cross-reactivity against other mouse heavy or light chain isotypes.

Purification: Immunoaffinity purified

Immunogen: Mouse IgG2b.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: Flo (1:2000 - 1:10000), IF (1:500 - 1:2500)

Usage: Suitable for use in Immunofluorescence microscopy and Flow Cytometry. Flow Cytometry: 1:2000-1:10000. Immunofluorescence: 1:500-1:2500. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile 40-50 % glycerol, dH2O.

Concentration: Lot specific

Buffer: Lyophilized in PBS,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide. Reconstitute in sterile 40-50 % glycerol, dH2O.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Product is photosensitive and should be protected from light.

Target Details

Target: IgG2b

Target Type: Antibody