Mouse anti-Rat IgM (Chain mu) Antibody

Catalog No. ABIN371247

Quick Overview for Mouse anti-Rat IgM (Chain mu) Antibody (ABIN371247)

Target: IgM

Clonality: Monoclonal

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Clone: M 2A1

Isotype: IgG1

Product Details

Binding Specificity: Chain mu

Reactivity: Rat

Host: Mouse

Purification: Purified

Application Details

Application Notes: Flow Cytometry and Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells. ELISA. Second step reagent for rat IgM Monoclonal Antibodies. Surface IgM Detection. B Cell Stimulation.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

Target Details

Target: IgM

Target Type: Antibody

Background: IgM normally constitutes about 10 % of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences. Class specific anti immunoglobulin antibodies are useful for: The characterization of malignant B cell proliferations. All but acute lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenström's disease bear intracytoplasmic IgM. The other isotypes are less frequently found. On the other hand multiple myelomas are usually of the IgG or IgA type. Characterization of plasma cells in inflammatory conditions: Plasma cell typing can be of use for the classification of intestinal inflammatory conditions such as inflammatory bowel disease and allergic conditions. In the latter a specific increase in the number of IgE plasma cells can be demonstrated.Synonyms: Rat Immunoglobulin M