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Rabbit anti-Rat IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP))

ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP) F(ab')2 fragment
Catalog No. ABIN375723

Quick Overview for Rabbit anti-Rat IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) (ABIN375723)

Target

IgG

Fragment

F(ab')2 fragment

Clonality

  • 3437
  • 229
  • 16
  • 1
Polyclonal

Conjugate

  • 665
  • 422
  • 422
  • 398
  • 273
  • 262
  • 235
  • 118
  • 105
  • 72
  • 67
  • 64
  • 61
  • 58
  • 58
  • 50
  • 44
  • 43
  • 40
  • 39
  • 39
  • 37
  • 35
  • 35
  • 32
  • 31
  • 31
  • 30
  • 29
  • 29
  • 29
  • 29
  • 29
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 27
  • 26
  • 26
  • 25
  • 21
  • 21
  • 19
  • 17
  • 17
  • 16
Alkaline Phosphatase (AP)

Application

  • 2117
  • 2049
  • 1885
  • 1872
  • 999
  • 920
  • 896
  • 520
  • 424
  • 234
  • 234
  • 149
  • 102
  • 98
  • 88
  • 84
  • 51
  • 33
  • 30
  • 28
  • 25
  • 20
  • 15
  • 11
  • 9
  • 8
  • 7
  • 5
  • 5
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype

IgG
  • Binding Specificity

    • 2396
    • 517
    • 417
    • 146
    • 130
    • 128
    • 66
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Heavy & Light Chain

    Reactivity

    • 752
    • 703
    • 607
    • 467
    • 410
    • 244
    • 213
    • 176
    • 146
    • 141
    • 124
    • 110
    • 107
    • 101
    • 82
    • 62
    • 47
    • 28
    • 27
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat

    Host

    • 1773
    • 1582
    • 567
    • 282
    • 122
    • 112
    • 41
    • 32
    • 22
    • 21
    • 15
    • 11
    • 11
    Rabbit

    Specificity

    Reacts with the heavy and light chains of rat IgG 1, IgG 2a, IgG 2b and IgG 2c, and with the light chains of rat IgM as demonstrated by ELISA and /or flow cytometry.

    Characteristics

    Source: Pepsin digest of rabbit anti-rat IgG (H+L).
    To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance to characteristics of a standard reference reagent.

    Purification

    Affinity chromatography on hamster IgG covalently linked to agarose. F(ab') 2 fragment prepared by pepsin digestion of affinity purified IgG.
  • Application Notes

    Working Dilution:
    1:2,000-1:4,000
    Representative data are included in this product insert.

    Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Handling Advice

    Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Do not add Sodium azide.
    Dilute only prior to immediate use
    Each reagent is stable for the period shown on the bottle label if stored as directed.

    Storage

    4 °C
  • Target

    IgG

    Target Type

    Antibody
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