Mouse anti-Human IgM Antibody (APC)

Catalog No. ABIN375808

Quick Overview for Mouse anti-Human IgM Antibody (APC) (ABIN375808)

Target: IgM

Clonality: Monoclonal

Conjugate: APC

Application: ELISA, Flow Cytometry (FACS)

Clone: SA-DA4

Isotype: IgG1

Product Details

Reactivity: Human

Host: Mouse

Specificity: Human IgM, Mr 900 kDa

Characteristics: Mouse Anti-Human IgM-APC

Purification: Purified

Immunogen: Human IgM myeloma protein

Application Details

Application Notes:

• Applications: ELISA - Quality tested , FLISA - Quality tested FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , WB - Reported in literature , Multiplex - Reported in literature , Stim - Reported in literature
• Working Dilutions: ELISA AP conjugate 1:1,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 10,000 FLISA FITC and AF488 conjugates 1:200 - 1:400 AF555 conjugate 1:100 - 1:400 PE, APC, and AF647 conjugates 1 g/mL Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells PE and APC conjugates 0.2 g/106 cells AF647 conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

Excitation/Emission wavelength: 650 nm/660 nm

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Magri, Comerma, Pybus, Sintes, Lligé, Segura-Garzón, Bascones, Yeste, Grasset, Gutzeit, Uzzan, Ramanujam, van Zelm, Albero-González, Vazquez, Iglesias, Serrano, Márquez, Mercade, Mehandru, Cerutti: "Human Secretory IgM Emerges from Plasma Cells Clonally Related to Gut Memory B Cells and Targets Highly Diverse Commensals." in: Immunity, Vol. 47, Issue 1, pp. 118-134.e8, (2017) (PubMed).

Target Details

Target: IgM

Target Type: Antibody