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Goat anti-Mouse IgG (Heavy & Light Chain) Antibody (Cy5) - Preadsorbed

ELISA, FACS Polyclonal IgG Cy5 F(ab')2 fragment
Catalog No. ABIN376046

Quick Overview for Goat anti-Mouse IgG (Heavy & Light Chain) Antibody (Cy5) - Preadsorbed (ABIN376046)

Target

IgG

Fragment

F(ab')2 fragment

Clonality

  • 3437
  • 230
  • 16
  • 1
Polyclonal

Conjugate

  • 665
  • 422
  • 422
  • 398
  • 274
  • 262
  • 235
  • 118
  • 105
  • 72
  • 67
  • 64
  • 61
  • 58
  • 58
  • 50
  • 44
  • 43
  • 39
  • 39
  • 39
  • 37
  • 35
  • 35
  • 32
  • 31
  • 31
  • 30
  • 29
  • 29
  • 29
  • 29
  • 29
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 27
  • 26
  • 26
  • 25
  • 21
  • 21
  • 19
  • 17
  • 17
  • 16
Cy5

Application

  • 2118
  • 2050
  • 1884
  • 1873
  • 999
  • 920
  • 896
  • 520
  • 424
  • 234
  • 234
  • 149
  • 102
  • 98
  • 88
  • 84
  • 51
  • 33
  • 30
  • 28
  • 25
  • 20
  • 15
  • 11
  • 9
  • 8
  • 7
  • 5
  • 5
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Flow Cytometry (FACS)

Isotype

IgG
  • Binding Specificity

    • 2396
    • 517
    • 417
    • 146
    • 130
    • 128
    • 66
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Heavy & Light Chain

    Reactivity

    • 753
    • 702
    • 607
    • 468
    • 410
    • 244
    • 213
    • 176
    • 146
    • 141
    • 124
    • 110
    • 107
    • 101
    • 82
    • 62
    • 47
    • 28
    • 27
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse

    Host

    • 1772
    • 1583
    • 567
    • 282
    • 122
    • 112
    • 41
    • 32
    • 22
    • 21
    • 16
    • 11
    • 11
    Goat

    Specificity

    Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3 and with the light chains of mouse IgM and IgA

    Characteristics

    Goat F(ab')2 Anti-Mouse IgG(H+L), Human ads-CY5

    Purification

    Preadsorption: Human adsorbed

  • Application Notes

    • Applications: Quality tested applications include - ELISA , FLISA FC ,
    • Other referenced applications include - ELISPOT , IHC-FS , IHC-PS , ICC , WB
    • Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC and AF488 conjugates 1:100 - 1:400 AF555 conjugate 1:50 - 1:200 PE, APC, and CY5 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, SPRD, and CY5 conjugates 0.1 g/106 cells PE/CY5.5 conjugate 0.05 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

    Comment

    Excitation / Emission wavelength: 652 nm / 672 nm

    Restrictions

    For Research Use only
  • Buffer

    0.25 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Protect conjugated products from light.
    Each reagent is stable for the period shown on the bottle label if stored as directed.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C
  • Target

    IgG

    Target Type

    Antibody
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