Goat anti-Mouse IgG2b (Heavy Chain) Antibody (Cy5)

Catalog No. ABIN376049

Quick Overview for Goat anti-Mouse IgG2b (Heavy Chain) Antibody (Cy5) (ABIN376049)

Target: IgG2b

Clonality: Polyclonal

Conjugate: Cy5

Application: Flow Cytometry (FACS)

Isotype: IgG

Product Details

Binding Specificity: Heavy Chain

Reactivity: Mouse

Host: Goat

Specificity: Reacts with the heavy chain of mouse IgG 2b as demonstrated by ELISA and flow cytometry.

Characteristics: Source: Pooled antisera from goats hyperimmunized with mouse IgG 2b paraproteins.
To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance to characteristics of a standard reference reagent.

Purification: Affinity chromatography on mouse IgG 2b covalently linked to agarose

Application Details

Application Notes: Working Dilution:
<= 01 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Comment:

Excitation / Emission wavelength: 652 nm / 672 nm

Restrictions: For Research Use only

Handling

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

References

Nelson, Nonnecke, Reinhardt, Waters, Beitz, Lippolis: "Regulation of Mycobacterium-specific mononuclear cell responses by 25-hydroxyvitamin D3." in: PLoS ONE, Vol. 6, Issue 6, pp. e21674, (2011) (PubMed).

Hardie, Baldwin, Naylor, Haworth, Hou, Lax, Curnow, Willcox, MacFadyen, Isacke, Buckley: "The stromal cell antigen CD248 (endosialin) is expressed on naive CD8+ human T cells and regulates proliferation." in: Immunology, Vol. 133, Issue 3, pp. 288-95, (2011) (PubMed).

Parsonage, Filer, Bik, Hardie, Lax, Howlett, Church, Raza, Wong, Trebilcock, Scheel-Toellner, Salmon, Lord, Buckley: "Prolonged, granulocyte-macrophage colony-stimulating factor-dependent, neutrophil survival following rheumatoid synovial fibroblast activation by IL-17 and TNFalpha." in: Arthritis research & therapy, Vol. 10, Issue 2, pp. R47, (2008) (PubMed).

Target Details

Target: IgG2b

Target Type: Antibody