Goat anti-Rabbit IgG (Heavy Chain) Antibody (HRP)

Catalog No. ABIN376288

Quick Overview for Goat anti-Rabbit IgG (Heavy Chain) Antibody (HRP) (ABIN376288)

Target: IgG

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: Heavy Chain

Reactivity: Rabbit

Host: Goat

Specificity: Reacts with the heavy chain of rabbit IgG as demonstrated by ELISA.

Characteristics: Source: Pooled antisera from goats hyperimmunized with normal rabbit IgG
To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance to characteristics of a standard reference reagent.

Purification: Affinity chromatography on rat IgG covalently linked to agarose

Application Details

Application Notes: Working Dilution:
1:4,000-1:8,000
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

References

Beauchemin, Moerke, Faloon, Kaye: "Assay Development and High-Throughput Screening for Inhibitors of Kaposi's Sarcoma-Associated Herpesvirus N-Terminal Latency-Associated Nuclear Antigen Binding to Nucleosomes." in: Journal of biomolecular screening, (2014) (PubMed).

Vistnes, Aronsen, Lunde, Sjaastad, Carlson, Christensen: "Pentosan Polysulfate Decreases Myocardial Expression of the Extracellular Matrix Enzyme ADAMTS4 and Improves Cardiac Function In Vivo in Rats Subjected to Pressure Overload by Aortic Banding." in: PLoS ONE, Vol. 9, Issue 3, pp. e89621, (2014) (PubMed).

Mau, Kaiser, Suedekum: "Carbonic anhydrase II is secreted from bovine parotid glands." in: Histology and histopathology, Vol. 25, Issue 3, pp. 321-9, (2010) (PubMed).

Basile, Friedrich, Spahic, Knipe, Mang, Leonard, Ashtiyani, Bacallao, Molitoris, Sutton: "Impaired endothelial proliferation and mesenchymal transition contribute to vascular rarefaction following acute kidney injury." in: American journal of physiology. Renal physiology, (2010) (PubMed).

Target Details

Target: IgG

Target Type: Antibody