Mouse anti-Human IgG (Fc Region) Antibody

Catalog No. ABIN376392

Quick Overview for Mouse anti-Human IgG (Fc Region) Antibody (ABIN376392)

Target: IgG

Clonality: Monoclonal

Application: ELISA, Flow Cytometry (FACS)

Clone: H2

Isotype: IgG2b

Product Details

Binding Specificity: Fc Region

Reactivity: Human

Host: Mouse

Specificity: Reacts with the Fc portion of the heavy chain of all subclasses of human IgG as demonstrated by ELISA,

Characteristics: Mouse Anti-Human IgG Fc-LE/AF

Purification: Purified

Endotoxin Level: Low endotoxin

Immunogen: Unknown

Application Details

Application Notes:

• Applications: ELISA - Quality tested , FLISA - Quality tested FC - Quality tested , ELISPOT - Reported in literature , WB - Reported in literature , Multiplex - Reported in literature
• Working Dilutions: ELISA AP conjugate 1:2,000 - 1:8,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:10,000 - 1:20,000 FLISA FITC conjugate 1:200 - 1:600 AF488 and AF555 conjugates 1:100 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells CY5 conjugate 0.3 g/106 cells PE, APC, SPRD, and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg purified immunoglobulin in 1.0 mL of PBS

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.

Target Details

Target: IgG

Target Type: Antibody