Goat anti-Mouse Ig Antibody (PE) - Preadsorbed

Catalog No. ABIN376432

Quick Overview for Goat anti-Mouse Ig Antibody (PE) - Preadsorbed (ABIN376432)

Target: Ig

Clonality: Polyclonal

Conjugate: PE

Application: ELISA, Flow Cytometry (FACS)

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Goat

Specificity: Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, and IgA

Characteristics: Goat Anti-Mouse Ig, Human ads-PE

Purification:

Purification Method: Affinity chromatography on mouse IgG, IgM, and IgA covalently linked to agarose.

Preadsorption: Human Adsorbed

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA , FLISA FC ,
• Other referenced applications include - ELISPOT , IHC-FS , IHC-PS , ICC , WB , Sep
• Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BGAL conjugate 1:500 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, TRITC, TXRD, AF488, and AF555 conjugates 1:100 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Gumber, Amancha, Yen, Villinger, Gabuzda, Byrareddy: "In vivo characterization of macrophage-tropic simian immunodeficiency virus molecular clones in rhesus macaques." in: Journal of neurovirology, Vol. 24, Issue 4, pp. 411-419, (2018) (PubMed).

Pool, Kol, de Jong, de Vries, Lub-de Hooge, Terwisscha van Scheltinga: "89Zr-mAb3481 PET for HER3 tumor status assessment during lapatinib treatment." in: mAbs, Vol. 9, Issue 8, pp. 1370-1378, (2018) (PubMed).

Geno, Kennedy, Sawyer, Brown, Nahm: "Ficolin-2 inhibitors are present in sera after prolonged storage at -80 °C." in: PeerJ, Vol. 4, pp. e2705, (2016) (PubMed).

Byrareddy, Little, Mayne, Villinger, Ansari: "Phenotypic and Functional Characterization of Monoclonal Antibodies with Specificity for Rhesus Macaque CD200, CD200R and Mincle." in: PLoS ONE, Vol. 10, Issue 10, pp. e0140689, (2016) (PubMed).

van Roosmalen, Reis, Setroikromo, Yuvaraj, Joseph, Tepper, Kruyt, Quax: "The ER stress inducer DMC enhances TRAIL-induced apoptosis in glioblastoma." in: SpringerPlus, Vol. 3, pp. 495, (2015) (PubMed).

Target Details

Target: Ig