Goat anti-Human IgM Antibody (PE)

Catalog No. ABIN376506

Quick Overview for Goat anti-Human IgM Antibody (PE) (ABIN376506)

Target: IgM

Clonality: Polyclonal

Conjugate: PE

Application: ELISA, Flow Cytometry (FACS)

Isotype: IgG

Product Details

Reactivity: Human

Host: Goat

Specificity: Reacts with the heavy chain of human IgM

Characteristics: Goat Anti-Human IgM-PE

Purification: Purification Method: Affinity chromatography on human IgM covalently linked to agarose.

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA , FLISA FC ,
• Other referenced applications include - ELISPOT , IHC-FS , IHC-PS , ICC , WB , IP , Multiplex , Stim , Purification , Depletion
• Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, TRITC, TXRD, AF488, and AF555 conjugates 1:100 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Ezzelarab, Zhang, Guo, Lu, Zahorchak, Wiseman, Nalesnik, Bhama, Cooper, Thomson: "Regulatory T Cell Infusion Can Enhance Memory T Cell and Alloantibody Responses in Lymphodepleted Nonhuman Primate Heart Allograft Recipients." in: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, Vol. 16, Issue 7, pp. 1999-2015, (2017) (PubMed).

Azimzadeh, Byrne, Ezzelarab, Welty, Braileanu, Cheng, Robson, McGregor, Cooper, Pierson: "Development of a consensus protocol to quantify primate anti-non-Gal xenoreactive antibodies using pig aortic endothelial cells." in: Xenotransplantation, Vol. 21, Issue 6, pp. 555-66, (2015) (PubMed).

Target Details

Target: IgM

Target Type: Antibody