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Goat anti-Human IgA Antibody (PE)

ELISA, FACS Polyclonal IgG PE F(ab')2 fragment

FLISA plate was coated with purified human IgA, IgG, and IgM. (Goat anti-Human IgA Antibody (PE))

1 image

(2 references)

Catalog No. ABIN376514

Quick Overview for Goat anti-Human IgA Antibody (PE) (ABIN376514)

Target

IgA

Fragment

F(ab')2 fragment

Clonality

Polyclonal

Conjugate

PE

Application

193
145
130
107
107
103
61
47
47
34
28
26
23
18
16
16
14
7
7
4
3
2
2
2
2
1
1
1
1
1

ELISA, Flow Cytometry (FACS)

Isotype

IgG

Reactivity
188

61

45

16

15

13

13

12

12

11

9

6

6

5

4

3

3

2

1

1

1

1
Human
Host
200

109

58

17

14

6

2
Goat

Specificity

Reacts with the heavy chain of human IgA

Characteristics

Goat F(ab')2 Anti-Human IgA-PE

Purification

Affinity chromatography on sheep IgG covalently coupled to agarose. Gel filtration chromatography of pepsin digested antibody.
Mouse anti-Human IgA (Heavy Chain) antibody
ELISA, WB, IHC (fro), IP Monoclonal Mc24-2E11 IgG1 unconjugated

Goat anti-Human IgA antibody
ELISA, FACS Polyclonal IgG unconjugated F(ab')2 fragment

Goat anti-Human IgA (Heavy Chain) antibody (Biotin)
ELISA, WB, FACS Polyclonal IgG Biotin

Goat anti-Human IgA (Heavy Chain) antibody (FITC)
ELISA, FACS Polyclonal IgG FITC

Goat anti-Human IgA (Heavy Chain) antibody (TRITC)
ELISA, FACS Polyclonal IgG TRITC

Goat anti-Human IgA antibody (Biotin)
ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment

Goat anti-Human IgA antibody (FITC)
ELISA, FACS Polyclonal IgG FITC F(ab')2 fragment

Goat anti-Human IgA (Heavy Chain) antibody (Texas Red (TR))
ELISA, FACS Polyclonal IgG Texas Red (TR)

Goat anti-Human IgA (Heavy Chain) antibody (Alkaline Phosphatase (AP))
ELISA, WB Polyclonal IgG Alkaline Phosphatase (AP)

Goat anti-Human IgA (Chain alpha) antibody (DyLight 650)
FACS, IF DyLight 650

Application Notes
- Applications: Quality tested applications include - ELISA , FLISA FC ,
- Other referenced applications include - ELISPOT , IHC-FS , Stim
- Working Dilutions: ELISA BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC conjugate 1:100 - 1:400 AF555 conjugate 1:200 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Restrictions

For Research Use only
Buffer

0.25 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage

4 °C

Storage Comment

Store at 2-8°C
Brandsma, Bondza, Evers, Koutstaal, Nederend, Jansen, Rösner, Valerius, Leusen, Ten Broeke: "Potent Fc Receptor Signaling by IgA Leads to Superior Killing of Cancer Cells by Neutrophils Compared to IgG." in: Frontiers in immunology, Vol. 10, pp. 704, (2019) (PubMed).

Magri, Comerma, Pybus, Sintes, Lligé, Segura-Garzón, Bascones, Yeste, Grasset, Gutzeit, Uzzan, Ramanujam, van Zelm, Albero-González, Vazquez, Iglesias, Serrano, Márquez, Mercade, Mehandru, Cerutti: "Human Secretory IgM Emerges from Plasma Cells Clonally Related to Gut Memory B Cells and Targets Highly Diverse Commensals." in: Immunity, Vol. 47, Issue 1, pp. 118-134.e8, (2017) (PubMed).
Target

IgA

Target Type

Antibody

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