Mouse anti-Human IgG (Fc Region) Antibody (PE)

Catalog No. ABIN376566

Quick Overview for Mouse anti-Human IgG (Fc Region) Antibody (PE) (ABIN376566)

Target: IgG

Clonality: Monoclonal

Conjugate: PE

Application: ELISA, Flow Cytometry (FACS)

Clone: JDC-10

Isotype: IgG1

Product Details

Binding Specificity: Fc Region

Reactivity: Human

Host: Mouse

Specificity: Reacts with the Fc portion of the heavy chain of all subclasses of human IgG as demonstrated by ELISA,

Characteristics: Mouse Anti-Human IgG Fc-PE

Purification: Purified

Immunogen: Purified human IgG1 myeloma protein

Application Details

Application Notes:

• Applications: ELISA - Quality tested , FLISA - Quality tested FC - Quality tested , IHC-PS - Reported in literature , WB - Reported in literature , Multiplex - Reported in literature
• Working Dilutions: ELISA AP conjugate 1:1,000 - 1:4,000 HRP conjugate 1:2,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA AF488 conjugate 1:200 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry Purified (UNLB) antibody 1 g/106 cells BIOT and AF488 conjugates 1 g/106 cells PE and AF647 conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Gilman, Furmanova-Hollenstein, Pascual, B van t Wout, Langedijk, McLellan: "Transient opening of trimeric prefusion RSV F proteins." in: Nature communications, Vol. 10, Issue 1, pp. 2105, (2019) (PubMed).

Chung, Nguyen, Lin, Lafrance-Vanasse, Scales, Lin, Deng, Williams, Sperinde, Li, Zheng, Sukumaran, Tesar, Ernst, Fischer, Lazar, Prabhu, Song: "An in vitro FcRn- dependent transcytosis assay as a screening tool for predictive assessment of nonspecific clearance of antibody therapeutics in humans." in: mAbs, Vol. 11, Issue 5, pp. 942-955, (2019) (PubMed).

Boesch, Kappel, Mahan, Chu, Crowley, Osei-Owusu, Alter, Ackerman: "Enrichment of high affinity subclasses and glycoforms from serum-derived IgG using FcγRs as affinity ligands." in: Biotechnology and bioengineering, Vol. 115, Issue 5, pp. 1265-1278, (2019) (PubMed).

Brown, Dowell, Boesch, Normandin, Mahan, Chu, Barouch, Bailey-Kellogg, Alter, Ackerman: "Multiplexed Fc array for evaluation of antigen-specific antibody effector profiles." in: Journal of immunological methods, Vol. 443, pp. 33-44, (2017) (PubMed).

Brown, Normandin, Osei-Owusu, Mahan, Chan, Lai, Vaccari, Rao, Franchini, Alter, Ackerman: "Microscale purification of antigen-specific antibodies." in: Journal of immunological methods, Vol. 425, pp. 27-36, (2016) (PubMed).

Brown, Licht, Dugast, Choi, Bailey-Kellogg, Alter, Ackerman: "High-throughput, multiplexed IgG subclassing of antigen-specific antibodies from clinical samples." in: Journal of immunological methods, Vol. 386, Issue 1-2, pp. 117-23, (2013) (PubMed).

Target Details

Target: IgG

Target Type: Antibody