Goat anti-Mouse IgG2b Antibody (PE-Cy7) - Preadsorbed

Catalog No. ABIN376634

Quick Overview for Goat anti-Mouse IgG2b Antibody (PE-Cy7) - Preadsorbed (ABIN376634)

Target: IgG2b

Clonality: Polyclonal

Conjugate: PE-Cy7

Application: ELISA, Flow Cytometry (FACS)

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Goat

Specificity: Reacts with the heavy chain of mouse IgG2b

Characteristics: Goat Anti-Mouse IgG2b, Human ads-PE/CY7

Purification:

Purification Method: Affinity chromatography on mouse IgG2b covalently linked to agarose.

Preadsorption: Human Adsorbed

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA , FLISA FC ,
• Other referenced applications include - ELISPOT , IHC-FS , IHC-PS , ICC , WB
• Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, TRITC, TXRD, AF488, and AF555 conjugates 1:100 - 1:400 PE, APC, CY5, and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, SPRD, CY5, PE/CY7, APC/CY7, and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Restrictions: For Research Use only

Handling

Buffer: 0.25 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Lei, Ryu, Wen, Twitchell, Bui, Ramesh, Weiss, Li, Samuel, Clark-Deener, Jiang, Lee, Yuan: "Increased and prolonged human norovirus infection in RAG2/IL2RG deficient gnotobiotic pigs with severe combined immunodeficiency." in: Scientific reports, Vol. 6, pp. 25222, (2018) (PubMed).

Target Details

Target: IgG2b

Target Type: Antibody