Goat anti-Mouse IgG2b (Heavy Chain) Antibody (TRITC)

Catalog No. ABIN376754

Quick Overview for Goat anti-Mouse IgG2b (Heavy Chain) Antibody (TRITC) (ABIN376754)

Target: IgG2b

Clonality: Polyclonal

Conjugate: TRITC

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Isotype: IgG

Product Details

Binding Specificity: Heavy Chain

Reactivity: Mouse

Host: Goat

Specificity: Reacts with the heavy chain of mouse IgG 2b as demonstrated by ELISA and flow cytometry.

Characteristics: Source: Pooled antisera from goats hyperimmunized with mouse IgG 2b paraproteins.
To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance to characteristics of a standard reference reagent.

Purification: Affinity chromatography on mouse IgG 1 covalently linked to agarose.

Application Details

Application Notes: Working Dilution:
<= 1 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

References

Song, Dohn, Rydeen, Nechiporuk, Waxman: "HDAC1-mediated repression of the retinoic acid-responsive gene ripply3 promotes second heart field development." in: PLoS genetics, Vol. 15, Issue 5, pp. e1008165, (2019) (PubMed).

Rydeen, Waxman: "Cyp26 Enzymes Facilitate Second Heart Field Progenitor Addition and Maintenance of Ventricular Integrity." in: PLoS biology, Vol. 14, Issue 11, pp. e2000504, (2017) (PubMed).

Menzl, Lebeau, Pandey, Hassounah, Li, Nagle, Weihs, McDermott: "Loss of primary cilia occurs early in breast cancer development." in: Cilia, Vol. 3, pp. 7, (2014) (PubMed).

Hassounah, Nagle, Saboda, Roe, Dalkin, McDermott: "Primary cilia are lost in preinvasive and invasive prostate cancer." in: PLoS ONE, Vol. 8, Issue 7, pp. e68521, (2013) (PubMed).

Lionnet, Czaplinski, Darzacq, Shav-Tal, Wells, Chao, Park, de Turris, Lopez-Jones, Singer: "A transgenic mouse for in vivo detection of endogenous labeled mRNA." in: Nature methods, Vol. 8, Issue 2, pp. 165-70, (2011) (PubMed).

Hardy, Filer, Cooper, Parsonage, Raza, Hardie, Rabbitt, Stewart, Buckley, Hewison: "Differential expression, function and response to inflammatory stimuli of 11beta-hydroxysteroid dehydrogenase type 1 in human fibroblasts: a mechanism for tissue-specific regulation of inflammation." in: Arthritis research & therapy, Vol. 8, Issue 4, pp. R108, (2007) (PubMed).

Target Details

Target: IgG2b

Target Type: Antibody