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Mouse anti-Human IgG (Fc Region) Antibody

ELISA, FACS Monoclonal H2 IgG2b unconjugated
Catalog No. ABIN376993

Quick Overview for Mouse anti-Human IgG (Fc Region) Antibody (ABIN376993)

Target

IgG

Clonality

  • 3110
  • 144
  • 6
  • 1
Monoclonal

Application

  • 1714
  • 1694
  • 1679
  • 1618
  • 921
  • 874
  • 799
  • 404
  • 348
  • 232
  • 232
  • 104
  • 87
  • 62
  • 51
  • 31
  • 24
  • 19
  • 18
  • 18
  • 16
  • 16
  • 12
  • 9
  • 8
  • 8
  • 5
  • 4
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Flow Cytometry (FACS)

Clone

H2

Isotype

IgG2b
  • Binding Specificity

    • 2333
    • 314
    • 286
    • 136
    • 133
    • 66
    • 53
    • 29
    • 28
    • 28
    • 19
    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Fc Region

    Reactivity

    • 631
    • 565
    • 550
    • 397
    • 360
    • 232
    • 190
    • 151
    • 134
    • 125
    • 111
    • 88
    • 86
    • 85
    • 82
    • 62
    • 47
    • 28
    • 27
    • 16
    • 15
    • 10
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human

    Host

    • 1538
    • 1381
    • 549
    • 203
    • 98
    • 84
    • 38
    • 25
    • 21
    • 20
    • 16
    • 11
    • 10
    Mouse

    Specificity

    Reacts with the Fc portion of the heavy chain of all subclasses of human IgG as demonstrated by ELISA,

    Characteristics

    Mouse Anti-Human IgG Fc-UNLB

    Purification

    Purified

    Immunogen

    Unknown
  • Application Notes

    • Applications: ELISA - Quality tested , FLISA - Quality tested FC - Quality tested , ELISPOT - Reported in literature , WB - Reported in literature , Multiplex - Reported in literature
    • Working Dilutions: ELISA AP conjugate 1:2,000 - 1:8,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:10,000 - 1:20,000 FLISA FITC conjugate 1:200 - 1:600 AF488 and AF555 conjugates 1:100 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells CY5 conjugate 0.3 g/106 cells PE, APC, SPRD, and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

    Preservative

    Without preservative

    Handling Advice

    Each reagent is stable for the period shown on the bottle label if stored as directed.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C
  • Target

    IgG

    Target Type

    Antibody
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