Mouse anti-Human IgE Antibody (Biotin)

Catalog No. ABIN457497

Quick Overview for Mouse anti-Human IgE Antibody (Biotin) (ABIN457497)

Target: IgE

Clonality: Monoclonal

Conjugate: Biotin

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)

Clone: Le27-NBS01

Isotype: IgG1 kappa

Product Details

Reactivity: Human

Host: Mouse

Specificity: Human IgE, Fce4, no cross-ractivity with other human or murine Ab isotypes

Sensitivity: Reactivity > 95 %, compared to calibrator (Internal Standard mAb clone Le27), tested by ELISA binding to immobilized human Standard IgE (clone SUS-11)

Characteristics: IGHE, chromosome 14q32.33

Purification: Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B - rat monoclonal anti- mouse Ck

Purity: > 95 %, tested by analytical Gel Permeation Chromatography

Immunogen: purified human myeloma IgE

Application Details

Application Notes: For functional cellular assays (mast cell / basophil activation), when used at dilutions < 1:100, the sodium azide free formulation (Cat. No. 0908-1-010-B) is recommended

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, pH=7.2, 0.1 % NaN3

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C

Target Details

Target: IgE

Background: The antibody recognizes an epitope within the Ce4 domain of human IgE. It does not block the binding of IgE to its high affinity receptor on mast cells and basophils and is therefore suitable to detect receptor bound IgE. It can also be used as cross-linking agent for allergen-independent triggering of mast cells and basophils.