Mouse anti-Human IgE Antibody (Biotin)

Catalog No. ABIN457499

Quick Overview for Mouse anti-Human IgE Antibody (Biotin) (ABIN457499)

Target: IgE

Clonality: Monoclonal

Conjugate: Biotin

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)

Clone: Le27-NBS01

Isotype: IgG1 kappa

Product Details

Reactivity: Human

Host: Mouse

Specificity: Human IgE, Fce4, no cross-ractivity with other human or murine Ab isotypes

Sensitivity: Reactivity > 95 %, compared to calibrator (Internal Standard mAb clone Le27), tested by ELISA binding to immobilized human Standard IgE (clone SUS-11)

Characteristics: IGHE, chromosome 14q32.33

Purification: Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B - rat monoclonal anti- mouse Ck

Purity: > 95 %, tested by analytical Gel Permeation Chromatography

Sterility: Sterile filtered

Immunogen: purified human myeloma IgE

Application Details

Application Notes: For functional cellular assays (mast cell / basophil activation), in particular when used at dilutions < 1:100

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, pH=7.2, sterile filtered, contains no NaN3

Preservative: Azide free

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C

Target Details

Target: IgE

Background: The antibody recognizes an epitope within the Ce4 domain of human IgE. It does not block the binding of IgE to its high affinity receptor on mast cells and basophils and is therefore suitable to detect receptor bound IgE. It can also be used as cross-linking agent for allergen-independent triggering of mast cells and basophils.