Goat anti-Mouse IgA, IgG, IgM (Fab Region) Antibody (FITC) - Preadsorbed

Catalog No. ABIN458392

Quick Overview for Goat anti-Mouse IgA, IgG, IgM (Fab Region) Antibody (FITC) - Preadsorbed (ABIN458392)

Target: IgA, IgG, IgM

Clonality: Polyclonal

Conjugate: FITC

Application: Immunofluorescence (IF)

Isotype: IgG

Product Details

Binding Specificity: Fab Region

Reactivity: Mouse

Host: Goat

Specificity: The reactivity of the antiserum is directed to the major isotypes of the mouse immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal mouse plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained.

Characteristics: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal goat antiserum to mouse IgG, IgA and IgM, heavy and light chains, adsorbed for human cross-reactivity.
Fluorochrome/IgG protein molar ratio (F/P) is approximately 1.7.

Purification: Preadsorption: Immunoaffinity adsorbed using insolubilized Ig-depleted mouse.

Immunogen: Purified polyclonal mouse IgG, and homogenous IgA and IgM isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: Direct immunofluorescence staining of monoclonal mouse monoclonal antibodies in the presence of human cell or serum components at the cellular and subcellular level. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.

Comment:

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water. Spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Concentration: 10 mg/mL

Buffer: FITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Handling Advice: Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgA, IgG, IgM

Alternative Name: IgG + IgA + IgM

Target Type: Antibody