Goat anti-Rat IgA Antibody (Rhodamine)

Catalog No. ABIN478706

Quick Overview for Goat anti-Rat IgA Antibody (Rhodamine) (ABIN478706)

Target: IgA

Clonality: Polyclonal

Conjugate: Rhodamine

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Isotype: IgA

Product Details

Reactivity: Rat

Host: Goat

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgA and Rat Serum. No reaction was observed against Rat IgG or Rat M. Specificity was confirmed by ELISA at less than 1 % cross reactivity against other Rat heavy or light chain isotypes.

Purification: Immunoaffinity purified

Immunogen: Rat IgA a-chain.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: Flo, IF

Usage: Suitable for use in Immunomicroscopy, Flow Cytometry and FACS. Optimal dilutions have not been tested. Label: Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons). Absorption Wavelength: 550nm. Emission Wavelength: 570nm. Fluorochrome/Protein Ratio: 2.5 Moles TRITC per mole of Goat IgG. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile 40-50 % glycerol

Concentration: Lot specific

Buffer: Lyophilized powder from PBS,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details

Target: IgA

Target Type: Antibody