Rabbit anti-Pig IgA Secretory Component Antibody
ID, IP
Polyclonal
unconjugated
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- Target
- IgA Secretory Component
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Reactivity
- Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Application
- Immunodiffusion (ID), Immunoprecipitation (IP)
- Purification
- Serum
- Immunogen
- Highly purified secretory IgA isolated from pooled Swine milk. Freund's complete adjuvant is used in the first step of the immunization procedure.
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- Application Notes
- Precipitation. Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in swine milk or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of swine IgA from serum or exocrine secretions. Antisera to IgA or sIgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude the use of the antiserum in more sensitive techniques if proper controls are included. Recommended Dilutions : Immunoelectrophoresis: Use 2 μL or equivalent against 120 μL antiserum. Double Radial Immunodiffusion: Use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody Titre Precipitin titre not less than 1/32 when tested against pooled normal swine serum in agar-block immunodiffusion titration.
- Restrictions
- For Research Use only
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- Storage
- 4 °C,-20 °C
- Storage Comment
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
- Expiry Date
- 12 months
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- Target
- IgA Secretory Component
- Alternative Name
- iga (secretory)
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