Goat anti-Rat IgA, IgG, IgM Antibody

Catalog No. ABIN5608123

Supplier Product No.: 612-101-130

Quick Overview for Goat anti-Rat IgA, IgG, IgM Antibody (ABIN5608123)

Target: IgA, IgG, IgM

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Reactivity: Rat

Host: Goat

Supplier Product No.: 612-101-130

Supplier: Rockland

Purpose: Rat IgG IgA IgM (H&L) Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgA and Rat IgM.  This reagent is suitable for the detection of all Rat immunoglobulin subclasses and chain combinations.

Characteristics: RAT IgG (H&L) (GOAT) Antibody was generated in goat detects specifically Rat IgG whole molecule. Anti-Rat IgG antibody is ideal for investigators involved in serum protein component research.

Purification: Goat-Anti-Rat IgG IgA IgM Antibody was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Optional[Immunogen]: Rat IgG, IgA and IgM whole molecules

Application Details

Application Notes: Application Note: Goat-Anti-Rat IgG IgA IgM antibody is suitable for use in various immunoassays. Specific conditions for reactivity and signal detection should be optimized by the end user. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

Target Details

Target: IgA, IgG, IgM

Alternative Name: IgG,IgA,IgM

Target Type: Antibody

Background: Rat IgM antibody recognizes the mu chain of the Rat IgM. No reaction was observed against Rat IgA or Rat IgG. ELISA was used to confirm specificity at less than 1 % cross reactivity against other rat heavy or light chain isotypes. Anti-Rat IgM antibody is ideal with investigators involved in serum component protein research.