Goat anti-Dog IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN611929

Quick Overview for Goat anti-Dog IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) (ABIN611929)

Target: IgG

Conjugate: Alkaline Phosphatase (AP)

Application: Immunoassay (IA)

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Dog

Host: Goat

Characteristics: Goat anti-Dog IgG (H&L) - Affinity Pure, ALP Conjugate.

Purification: Affinity purified using solid phase cat IgG (H&L)

Purity: > 95 % based on SDS-PAGE

Immunogen: Purified Dog IgG, whole molecule

Application Details

Application Notes: This conjugate is suitable for all all immunoassays applications.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:500-1:2,000 for Immunohistochemistry, 1:50,000-1:5,000 for ELISA/Western blot

Comment:

Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

ALP: Enzyme derived from calf intestine (U.S. origin), Catalyzes the hydrolysis of phosphate groups from a substrate, which produces a colored reaction product or a release of light, The most common substrate for ALP is pNPP, which produces a soluble product, Unaffected by biological components in the ELISA assay, and produces consistent results, The reaction rate of ALP is linear, so its detection sensitivity can be increased by increasing the length of the reaction time. Detection OD of 405 nm

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.5 mg/mL

Buffer: 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Target Details

Target: IgG

Target Type: Antibody