Mouse anti-Human IgE Antibody (PE)

Catalog No. ABIN616797

Quick Overview for Mouse anti-Human IgE Antibody (PE) (ABIN616797)

Target: IgE

Clonality: Monoclonal

Conjugate: PE

Application: Flow Cytometry (FACS)

Clone: BE5

Isotype: IgG1

Product Details

Reactivity: Human

Host: Mouse

Cross-Reactivity (Details): Species reactivity (tested):Human

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Excitation/Emission wavelength: 496 nm/576 nm

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Product is photosensitive and should be protected from light.

Target Details

Target: IgE

Background: Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.Synonyms: Immunoglobulin E human