Goat anti-Human IgG (Heavy & Light Chain) Antibody (TRITC)

Catalog No. ABIN619739

Quick Overview for Goat anti-Human IgG (Heavy & Light Chain) Antibody (TRITC) (ABIN619739)

Target: IgG

Fragment: F(ab')2 fragment

Clonality: Polyclonal

Conjugate: TRITC

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Human

Host: Goat

Characteristics: Goat anti-human IgG (H&L), F(ab) '2 fragment is a secondary, Rhodamine (TRITC-rhodamine - Tetramethylrhodamine-5-isothiocyanate) conjugated antibody, which is reacting against human IgG (H&L), F(ab) '2 fragment. TRITC has Amax = 550 nm, Emax = 570 nm. Antibody solution may contain small amounts of intact IgG.
F(ab)'2 fragment

Purification: Affinity purification

Purity: > 90 % based on SDS-PAGE.

Application Details

Application Notes: Needs to be determined by end user for immunocytochemistry (ICC), immunohistochemistry (IHC) and for flow cytometry.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution please add 0.55 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.

Handling Advice: Product is photosensitive and should be protected from light.

Storage: 4 °C

Storage Comment: Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Target Details

Target: IgG

Target Type: Antibody