Donkey anti-Human IgG Antibody (Cy2) - Preadsorbed

Catalog No. ABIN6698908

Supplier Product No.: 609-711-123

Product Details

Target: IgG

Reactivity: Human

Host: Donkey

Clonality: Polyclonal

Conjugate: Cy2

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Supplier Product No.: 609-711-123

Supplier: Rockland

Purpose: Human IgG (H&L) Antibody CY2 Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Ms Rb Rt & Sh Serum Proteins

Characteristics: Donkey Anti Human IgG CY2 Conjugated Antibody, Donkey Anti-Human IgG Antibody CY2 Antibody,Anti-Human IgG (H&L) Cy2 generated in donkey detects human Immunoglobulin G (heavy and light chains).

Purification: Preadsorption: Pre-Adsorbed

Immunogen: Human IgG whole molecule

Isotype: IgG

Application Details

Application Notes:

FLISA_Dilution: 1:10,000 - 1:50,000

ELISA_Dilution: 1:10,000 - 1:50,000

Flow_Cytometry_Dilution: 1:500 - 1:2,500

IF_Microscopy_Dilution: 1:1,000 - 1:5,000

Western_Blot_Dilution: 1:1,000 - 1:5,000

Comment:

Cy2 Conjugated Secondary Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.