Goat anti-Mouse IgG Antibody (Cy3) - Preadsorbed

Catalog No. ABIN6698986

Supplier Product No.: 610-104-121

Product Details

Target: IgG

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: Cy3

Application: Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Supplier Product No.: 610-104-121

Supplier: Rockland

Purpose: Mouse IgG (H&L) Antibody CY3 Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins

Characteristics: Goat Anti-Mouse IgG (H&L) Antibody CY3 Conjugated Pre-Adsorbed, Goat Anti Mouse IgG Antibody CY3 Conjugated,Anti-Mouse IgG Cy3 Antibody generated in goat detects reactivity to Mouse IgG.

Purification: Preadsorption: Pre-Adsorbed

Immunogen: Mouse IgG whole molecule

Isotype: IgG

Labeling Ratio: 8.5

Application Details

Application Notes:

FLISA_Dilution: 1:10,000 - 1:50,000

Flow_Cytometry_Dilution: 1:500 - 1:2,500

IF_Microscopy_Dilution: 1:1,000 - 1:5,000

Other: FLOW CYTOMETRY 1:500 - 1:2,500

Comment:

Anti-Mouse IgG Cy3 Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Suggested Applications: IF, IHC, Multiplex

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Ng-Blichfeldt, Stewart, Clatworthy, Williams, Röper: "Identification of a core transcriptional program driving the human renal mesenchymal-to-epithelial transition." in: Developmental cell, Vol. 59, Issue 5, pp. 595-612.e8, (2024) (PubMed).

Hartmann, Raabe, Wenisch, Arnhold: "Amniotic fluid derived stem cells give rise to neuron-like cells without a further differentiation potential into retina-like cells." in: American journal of stem cells, Vol. 2, Issue 2, pp. 108-18, (2013) (PubMed).

Venkatesan, Natarajan, Schwarz, Mayer, Alpadi, Magupalli, Sung, Schmitz: "Nicotinamide adenine dinucleotide-dependent binding of the neuronal Ca2+ sensor protein GCAP2 to photoreceptor synaptic ribbons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 19, pp. 6559-76, (2010) (PubMed).

Arnhold, Post, Glüer, Hoopmann, Wenisch, Volpers, Addicks: "Neuronal characteristics of amniotic fluid derived cells after adenoviral transformation." in: Cell biology international, Vol. 32, Issue 12, pp. 1559-66, (2009) (PubMed).

Pyo, Sui, Dhume, Palomeque, Blaxall, Diaz, Tunstead, Logothetis, Hajjar, Schecter: "CXCR4 modulates contractility in adult cardiac myocytes." in: Journal of molecular and cellular cardiology, Vol. 41, Issue 5, pp. 834-44, (2007) (PubMed).

Arnhold, Klein, Klinz, Absenger, Schmidt, Schinköthe, Brixius, Kozlowski, Desai, Bloch, Addicks: "Human bone marrow stroma cells display certain neural characteristics and integrate in the subventricular compartment after injection into the liquor system." in: European journal of cell biology, Vol. 85, Issue 6, pp. 551-65, (2007) (PubMed).

Wolter, Tauer, Fuchs, Volk: "Mapping of cytoskeletal components in the hippocampal formation of the tree shrew (Tupaia belangeri)." in: Journal of chemical neuroanatomy, Vol. 17, Issue 2, pp. 65-74, (1999) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.