Goat anti-Mouse IgG (Fc Region) Antibody (DyLight 488)

Catalog No. ABIN6699046

Supplier Product No.: 610-141-003

Quick Overview for Goat anti-Mouse IgG (Fc Region) Antibody (DyLight 488) (ABIN6699046)

Target: IgG

Clonality: Polyclonal

Conjugate: DyLight 488

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotype: IgG

Product Details

Binding Specificity: Fc Region

Reactivity: Mouse

Host: Goat

Supplier Product No.: 610-141-003

Supplier: Rockland

Purpose: Mouse IgG Fc Antibody DyLight™ 488 Conjugated

Characteristics: Goat Anti Mouse IgG F(c) Antibody DyLight™ 488 Conjugated, Goat Anti-Mouse IgG Fc Antibody DyLight™ 488 Conjugated, Goat Anti Mouse IgG Fc Fragment Antibody DyLight™ 488 Conjugated,Anti-Mouse IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .

Immunogen: Mouse IgG F(c) fragment

Labeling Ratio: 3.1

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Comment:

Anti-Mouse IgG F(c) DyLight 488 has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: WB

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Rives, Vol, Fukazawa, Tinel, Trinquet, Ayoub, Shigemoto, Pin, Prézeau: "Crosstalk between GABAB and mGlu1a receptors reveals new insight into GPCR signal integration." in: The EMBO journal, Vol. 28, Issue 15, pp. 2195-208, (2010) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.