Goat anti-Rabbit IgG Antibody (DyLight 680) - Preadsorbed

Catalog No. ABIN6699115

Supplier Product No.: 611-144-122

Quick Overview for Goat anti-Rabbit IgG Antibody (DyLight 680) - Preadsorbed (ABIN6699115)

Target: IgG

Clonality: Polyclonal

Conjugate: DyLight 680

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotype: IgG

Product Details

Reactivity: Rabbit

Host: Goat

Supplier Product No.: 611-144-122

Supplier: Rockland

Purpose: Rabbit IgG (H&L) Antibody DyLight™ 680 Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins

Characteristics: Goat Anti-Rabbit IgG Antibody DyLight 680™ Conjugated, Goat Anti Rabbit IgG DyLight 680™ Conjugated Antibody,Anti-Rabbit IgG Antibody DyLight™680 generated in goat detects rabbit IgG.

Purification: Preadsorption: Pre-Adsorbed

Immunogen: Rabbit IgG whole molecule

Labeling Ratio: 2.8

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Comment:

Anti-Rabbit IgG Antibody DyLight™680 has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: FISH, Multiplex, WB

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Grossmann, Malburg, Glaß, Weeren, Sondermann, Pfeiffer, Petters, Lukas, Seibler, Klein, Grünewald, Hermann: "Mitochondria-Endoplasmic Reticulum Contact Sites Dynamics and Calcium Homeostasis Are Differentially Disrupted in PINK1-PD or PRKN-PD Neurons." in: Movement disorders : official journal of the Movement Disorder Society, Vol. 38, Issue 10, pp. 1822-1836, (2023) (PubMed).

White, Sun, Sauge, Cox, Donen, Pechkovsky, Straub, Francis, Bernatchez: "Limb-girdle muscular dystrophy type 2B causes HDL-C abnormalities in patients and statin-resistant muscle wasting in dysferlin-deficient mice." in: Skeletal muscle, Vol. 12, Issue 1, pp. 25, (2022) (PubMed).

Tao, Zhu, Diaz-Perez, Yu, Foley, Stewart, Casero, Steet, Zhai: "Phenylbutyrate modulates polyamine acetylase and ameliorates Snyder-Robinson syndrome in a Drosophila model and patient cells." in: JCI insight, Vol. 7, Issue 13, (2022) (PubMed).

Navarro, Tapia-Galisteo, Martín-García, Tarín, Corbacho, Gómez-López, Sánchez-Tirado, Campuzano, González-Cortés, Yáñez-Sedeño, Compte, Álvarez-Vallina, Sanz: "TGF-β-induced IGFBP-3 is a key paracrine factor from activated pericytes that promotes colorectal cancer cell migration and invasion." in: Molecular oncology, Vol. 14, Issue 10, pp. 2609-2628, (2021) (PubMed).

Zhang, Xu, Li, Yang, Sun, Wu, Qiao: "High iodine effects on the proliferation, apoptosis, and migration of papillary thyroid carcinoma cells as a result of autophagy induced by BRAF kinase." in: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Vol. 120, pp. 109476, (2020) (PubMed).

Asthana, Stern, Tang, Bugga, Li, Ferguson, Asthana, Bao, Drezek: "Development of a Novel Class of Self-Assembling dsRNA Cancer Therapeutics: A Proof-of-Concept Investigation." in: Molecular therapy oncolytics, Vol. 18, pp. 419-431, (2020) (PubMed).

Bodor, Valente, Mata, Black, Jansen: "Assembly in G1 phase and long-term stability are unique intrinsic features of CENP-A nucleosomes." in: Molecular biology of the cell, Vol. 24, Issue 7, pp. 923-32, (2013) (PubMed).

Chacko, Liberante, Paul, Longley, Fennell: "Voltage dependent anion channel-1 regulates death receptor mediated apoptosis by enabling cleavage of caspase-8." in: BMC cancer, Vol. 10, pp. 380, (2011) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG (H&L) is conjugated to DyLight™680.