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Recombinant Alpaca anti-Chicken IgY Antibody (Alexa Fluor 568)

IHC, FACS, IF, WB, ICC Monoclonal 3G2 Recombinant Antibody Alexa Fluor 568 single-domain Antibody (sdAb)
Catalog No. ABIN7272911

Quick Overview for Recombinant Alpaca anti-Chicken IgY Antibody (Alexa Fluor 568) (ABIN7272911)

Target

IgY

Antibody Type

Recombinant Antibody

Fragment

single-domain Antibody (sdAb)

Clonality

  • 102
  • 49
Monoclonal

Conjugate

  • 21
  • 13
  • 11
  • 10
  • 6
  • 6
  • 6
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  • 2
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  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Alexa Fluor 568

Application

  • 115
  • 108
  • 105
  • 98
  • 43
  • 36
  • 10
  • 6
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Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunocytochemistry (ICC)

Clone

3G2
  • Reactivity

    • 159
    • 37
    • 3
    • 2
    Chicken

    Host

    • 67
    • 59
    • 44
    • 19
    • 5
    • 4
    • 1
    Alpaca

    Expression System

    E.coli

    Specificity

    Chicken IgY, does not cross-react with mouse, rabbit, guinea pig or rat immunglobulins

    Purification

    Produced in: E.coli

    Immunogen

    anti-Chicken is a species-specific FluoTag directed against immunoglobulins from Chicken.

    Labeling Ratio

    Two site-specifically conjugated fluorophores per sdAb.
  • Application Notes

    IF: 1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed bellow)

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 2.5 µM

    Buffer

    The single sdAb clone was lyophilized from PBS pH 7.4.

    Storage

    4 °C/-20 °C/-80 °C

    Storage Comment

    Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
  • Target

    IgY

    Target Type

    Antibody

    Background

    The immune repertoire of chickens is much simpler compared to the one of mammals, producing only one isotype of immunoglobulins (IgY) with no subclasses. The use of polyclonal antibodies raised in chicken has steadily grown over the last years, mainly due to the need to add a new “species” when performing multitarget immunofluorescence.
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