Recombinant Alpaca anti-Chicken IgY Antibody (Alexa Fluor 568)

Catalog No. ABIN7272911

Quick Overview for Recombinant Alpaca anti-Chicken IgY Antibody (Alexa Fluor 568) (ABIN7272911)

Target: IgY

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Clonality: Monoclonal

Conjugate: Alexa Fluor 568

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunocytochemistry (ICC)

Clone: 3G2

Product Details

Reactivity: Chicken

Host: Alpaca

Expression System: E.coli

Specificity: Chicken IgY, does not cross-react with mouse, rabbit, guinea pig or rat immunglobulins

Purification: Produced in: E.coli

Immunogen: anti-Chicken is a species-specific FluoTag directed against immunoglobulins from Chicken.

Labeling Ratio: Two site-specifically conjugated fluorophores per sdAb.

Application Details

Application Notes: IF: 1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed bellow)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 2.5 µM

Buffer: The single sdAb clone was lyophilized from PBS pH 7.4.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Target Details

Target: IgY

Target Type: Antibody

Background: The immune repertoire of chickens is much simpler compared to the one of mammals, producing only one isotype of immunoglobulins (IgY) with no subclasses. The use of polyclonal antibodies raised in chicken has steadily grown over the last years, mainly due to the need to add a new “species” when performing multitarget immunofluorescence.