Recombinant Alpaca anti-Mouse IgG Antibody (Alexa Fluor 568)

Catalog No. ABIN7272914

Quick Overview for Recombinant Alpaca anti-Mouse IgG Antibody (Alexa Fluor 568) (ABIN7272914)

Target: IgG

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Clonality: Monoclonal

Conjugate: Alexa Fluor 568

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 1A23

Product Details

Reactivity: Mouse

Host: Alpaca

Expression System: E.coli

Specificity: Mouse IgG (Kappa light chain), does not cross-react with rabbit, guinea pig or chicken immunoglobulins

Purification: Produced in: E.coli

Immunogen: Our sdAbs anti-mouse KLC specifically binds to all mouse Ig isotypes carrying a kappa light chain.

Labeling Ratio: Two site-specifically conjugated fluorophores per sdAb.

Application Details

Application Notes: IF: 1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed bellow)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 2.5 µM

Buffer: The single sdAb clone was lyophilized from PBS pH 7.4.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Target Details

Target: IgG

Target Type: Antibody

Background: In most research monoclonal antibodies are generated in mice. These animals have a rich repertoire of immunoglobulins (Ig) displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mice IgGs can be further categorized into five subclasses IgG1, IgG2a, IgG2b, IgG2c, and IgG3. In addition to the subclasses, each IgG molecule can be equipped with either a lambda or kappa light chain.