Recombinant Alpaca anti-Mouse IgG Antibody (DBCO)
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- Target See all IgG products
- IgG
- Antibody Type
- Recombinant Antibody
- Fragment
- single-domain Antibody (sdAb)
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Reactivity
- Mouse
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Host
- Alpaca
- Expression System
- E.coli
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Clonality
- Monoclonal
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Conjugate
- DBCO
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- Mouse IgG (Kappa light chain), does not cross-react with rabbit, guinea pig or chicken immunoglobulins
- Purification
- Produced in: E.coli
- Immunogen
- sdAbs anti-mouse KLC specifically binds to all mouse Ig isotypes carrying a kappa light chain.
- Clone
- 1A23
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconsitute with 250 µL pure H20 for immidate use or with 250 µL 50 % glycerol for storage at -80 °C
- Buffer
- sdAbs are lyophilized from PBS pH 7.4.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
- Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
- Most research monoclonal antibodies are generated in mice. These animals have a rich repertoire of immunoglobulins (Ig) displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mice IgGs can be further categorized into five subclasses IgG1, IgG2a, IgG2b, IgG2c, and IgG3. In addition to the subclasses, each IgG molecule can be equipped with either a lambda, or kappa light chain.
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