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Mouse anti-Human IgG (Fab Region) Antibody

ELISA, WB, FACS Monoclonal 4A11 IgG1 kappa unconjugated
Catalog No. ABIN94400

Quick Overview for Mouse anti-Human IgG (Fab Region) Antibody (ABIN94400)

Target

IgG

Clonality

  • 3016
  • 144
  • 6
  • 1
Monoclonal

Application

  • 1686
  • 1672
  • 1639
  • 1568
  • 920
  • 873
  • 753
  • 407
  • 345
  • 232
  • 232
  • 106
  • 87
  • 71
  • 57
  • 31
  • 24
  • 19
  • 18
  • 18
  • 16
  • 16
  • 12
  • 9
  • 8
  • 7
  • 5
  • 4
  • 4
  • 4
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Clone

4A11

Isotype

IgG1 kappa
  • Binding Specificity

    • 2243
    • 315
    • 286
    • 149
    • 130
    • 67
    • 52
    • 29
    • 28
    • 28
    • 21
    • 5
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Fab Region

    Reactivity

    • 611
    • 547
    • 531
    • 395
    • 344
    • 231
    • 186
    • 154
    • 130
    • 124
    • 109
    • 86
    • 84
    • 82
    • 82
    • 62
    • 47
    • 28
    • 27
    • 16
    • 15
    • 10
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human

    Host

    • 1482
    • 1364
    • 523
    • 206
    • 98
    • 83
    • 38
    • 29
    • 21
    • 20
    • 16
    • 11
    • 11
    Mouse

    Purpose

    Anti-Hu IgG (Fab) Purified

    Specificity

    The mouse monoclonal antibody 4A11 reacts with Fab fragment of human IgG. This antibody detects Fab fragments regardless light chain type.

    Cross-Reactivity (Details)

    Human

    Purification

    Purified by protein-A affinity chromatography.

    Purity

    > 95 % (by SDS-PAGE)

    Immunogen

    Purified human IgG.
  • Application Notes

    Flow cytometry: Recommended dilution: 1-5 μg/mL.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Do not freeze.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C. Do not freeze.
  • Target

    IgG

    Target Type

    Antibody

    Background

    Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75 % of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.,Immunoglobulin G Fab fragment

    Molecular Weight

    150 kDa
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