Goat anti-Llama IgG (Chain gamma), (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN969730

Quick Overview for Goat anti-Llama IgG (Chain gamma), (Heavy & Light Chain) Antibody (HRP) (ABIN969730)

Target: IgG

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Product Details

Binding Specificity: Chain gamma, Heavy & Light Chain

Reactivity: Llama

Host: Goat

Specificity: Based on IEP, this antibody reacts with: heavy (gamma) chains on llama IgG, light chains on all llama immunoglobulins

Purification: Affinity purified using solid phase Llama IgG (H and L)

Purity: > 95 % based on SDS-PAGE

Immunogen: Purified Llama IgG, whole molecule

Application Details

Application Notes: This conjugate is suitable for all immunoassay applications.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:500-1:5,000 for Immunohistochemistry. 1:200-1:5000 for ELISA/Western blot

Comment:

Country of Origin: Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
HRP: Enzyme derived from the plant horseradish, A small molecule that does not cause steric hindrance in antibody-antigen reactions, Highly sensitive, can detect low levels of antibody and can be used in a sensitive ECL assay, Hydrogen peroxide oxidizes chromogenic substrates such as TMB, ABTS, and OPD to produce a soluble product with a distinct color which can be measured by spectrophotometer, The reaction product has a high color stability after the reaction is stopped. Detection OD of 450 nm

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Rehydrate with 1.1 mL of deionized water. Allow reconstituted product to stand for at least 30 minutes at room temperature prior to dilution. If necessary, Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Concentration: 1.0 mg/mL

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free

Preservative: Kathon CG

Handling Advice: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

Target Details

Target: IgG

Target Type: Antibody