Normal Mouse Serum
BI, ELISA, ICC, IF, IHC
Host: Mouse
Catalog No. ABIN7232260
Quick Overview for Normal Mouse Serum (ABIN7232260)
Target
Normal Serum
Host
Mouse
Application
BioImaging (BI), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
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Purpose
- Normal Mouse Serum
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Purification
- Lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2
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Purity
- Whole serum
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Application Notes
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-p (1:10-20), IF/ICC (1:10-20) and ELISA (1:100).
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Comment
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Blocking Serum
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- 10 mM sodium phosphate, 0.15 M sodium chloride with 0.05 % sodium azide as preservative, pH 7.2
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 2-8°C or aliquot and store at or below -20°C for long-term storage. Avoid repeated freezing and thawing.
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- Normal Serum
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Background
- High quality Normal mouse serum processed from non-immunized normal adult mouses is used as a blocking agent or as a negative control for immunoassays.,Normal mouse serums, which are from non-immuned mouse hosts, are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide. Normal serum diluted to 5 % -20 % (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific binding, or control for most immunoassay applications. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.,Serum
Target
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