WASH3P Protein (AA 1-463) (His tag)
custom-made
WASH3P
Origin: Human
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target
- WASH3P (WAS Protein Family Homolog 3 Pseudogene (WASH3P))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-463
- Origin
- Human
- Source
- HEK-293 Cells
- Purification tag / Conjugate
- This WASH3P protein is labelled with His tag.
- Purpose
- Predefined custom protein recombinant WASH3P Protein expressed in mammalian cells.
- Sequence
- MTPVRMQHSL AGQTYAVPLI QPDLRREEAV QQMADALQYL QKVSGDIFSR ISQQVEQSRS QVQAIGEKVS LAQAKIEKIK GSKKAIKVFS SAKYPAPERL QEYGSIFTGA QDPGLQRRPR HRIQSKHRPL DERALQEKDF PVCVSTKPEP EDDAEEGLGG LPSNISSVSS LLLFNTTENL GKKYVFLDPL AGAVTKTHVM LGAETEEKLF DAPLSISKRE QLEQQVPENY FYVPDLGQVP EIDVPSYLPD LPGITNDLMY IADLGPGIAP SAPGTIPELP TFHTEVAEPL KVDLQDGVLT PPPPPPPPPP APEVLASAPP LPPSTAAPVG QGARQDDSSS SASPSVQGAP REVVDPSGGR ATLLESIRQA GGIGKAKLRS MKERKLEKKQ QKEQEQVRAT SQGGHLMSDL FNKLVMRRKG ISGKGPGAGE GPGGAFARVS DSIPPLPPPQ QPQAEEDEDD WES Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- WASH3P (WAS Protein Family Homolog 3 Pseudogene (WASH3P))
- Alternative Name
- WASH3P
- Background
- Putative WAS protein family homolog 3 (Protein FAM39DP),FUNCTION: Acts as a nucleation-promoting factor at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting (PubMed:18159949, PubMed:20175130). Involved in endocytic trafficking of EGF (PubMed:20175130). Involved in transferrin receptor recycling. Regulates the trafficking of endosomal alpha5beta1 integrin to the plasma membrane and involved in invasive cell migration (By similarity). In T-cells involved in endosome-to-membrane recycling of receptors including T-cell receptor (TCR), CD28 and ITGAL, proposed to be implicated in T cell proliferation and effector function. In dendritic cells involved in endosome-to-membrane recycling of major histocompatibility complex (MHC) class II probably involving retromer and subsequently allowing antigen sampling, loading and presentation during T-cell activation. Involved in Arp2/3 complex-dependent actin assembly driving Salmonella typhimurium invasion independent of ruffling (By similarity). Involved in the exocytosis of MMP14 leading to matrix remodeling during invasive migration and implicating late endosome-to-plasma membrane tubular connections and cooperation with the exocyst complex (By similarity). Involved in negative regulation of autophagy independently from its role in endosomal sorting by inhibiting BECN1 ubiquitination to inactivate PIK3C3/Vps34 activity (By similarity). {ECO:0000250|UniProtKB:A8K0Z3, ECO:0000250|UniProtKB:Q8VDD8, ECO:0000269|PubMed:18159949, ECO:0000269|PubMed:20175130}.
- Molecular Weight
- 50.0 kDa
- UniProt
- C4AMC7
- AlphaFold
- C4AMC7
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