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Rabbit anti-Human IgG (F(ab')2 Region) Antibody

Details for Product No. ABIN101627, Supplier: Log in to see
Antigen
Epitope
F(ab')2 Region
4518
1867
1732
961
643
277
203
56
35
34
31
8
8
5
3
3
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
2468
2359
2055
1545
1279
628
581
401
328
315
315
309
293
224
164
112
105
76
32
24
21
13
12
8
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
Host
Rabbit
6100
4267
1527
605
545
298
85
49
43
8
8
4
4
3
1
1
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
Supplier
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Immunogen Anti-Human IgG fragment was produced in rabbit by repeated immunization with human IgG fragment.
Isotype IgG
Specificity IgG F(ab')2
Characteristics Anti-Human IgG F(ab')2 antibody detects human IgG F(ab')2 fragments. This secondary antibody anti-Human IgG F(ab')2 is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Human IgG F(ab')2 Antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.
Concentration Definition: by UV absorbance at 280 nm
Purification This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG, Human IgG F (ab')2 and Human Serum.
Sterility Sterile filtered
Background Synonyms: F(ab')2 Human IgG Antibody, Rb-a-Human F(ab')2 IgG, F(ab')2 Human IgG Antibody in Rabbit, Rabbit IgG Secondary Antibody.
Research Area Immunology, Secondary Antibodies
Application Notes Anti-Human IgG F(ab') 2 antibody is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Restrictions For Research Use only
Format Liquid
Concentration 2.2 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Background publications Titus, Haugland, Sharrow et al.: "Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies." in: Journal of immunological methods, Vol. 50, Issue 2, pp. 193-204, 1982 (PubMed).