Rabbit anti-Human IgG (F(ab')2 Region) Antibody - Preadsorbed

Catalog No. ABIN101627

Product Details

Target: IgG

Binding Specificity: F(ab')2 Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: IgG F(ab')2

Characteristics: Anti-Human IgG F(ab')2 antibody detects human IgG F(ab')2 fragments. This secondary antibody anti-Human IgG F(ab')2 is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Human IgG F(ab')2 Antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.
Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Human IgG F(ab')2 fragment was produced in rabbit by repeated immunization with human IgG F(ab')2 fragment.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Human IgG F(ab')2 antibody is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

ELISA Dilution: 1:350,000

Western Blot Dilution: 1:3,000 - 1:15,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

References

Kameoka, Kishi, Koura, Yamakawa, Nagasawa, Ito, Matsuda, Suzuki, Nakayama, Suzuki: "Efficacy of a recombinant single-chain fragment variable region, VasSF, as a new drug for vasculitis." in: Drug design, development and therapy, Vol. 13, pp. 555-568, (2019) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: F(ab')2 Human IgG Antibody, Rb-a-Human F(ab')2 IgG, F(ab')2 Human IgG Antibody in Rabbit, Pre-Adsorbed Human IgG Secondary Antibody

Background: Anti-Human IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the human IgG molecule. Human IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind human IgG F(c) will not bind human IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Human IgG F(ab')2 Antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.