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Rabbit anti-Human IgG (F(ab')2 Region) Antibody

Details for Product No. ABIN101627, Supplier: Log in to see
Antigen
Epitope
F(ab')2 Region
4385
2530
1763
1035
924
824
306
283
35
34
31
8
6
5
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
2656
2626
2286
1633
1385
738
607
422
365
337
333
321
309
259
165
147
124
112
41
27
25
21
12
11
9
8
6
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Host
Rabbit
6522
4533
1762
768
636
365
93
51
50
9
8
5
4
3
1
1
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Immunogen Anti-Human IgG fragment was produced in rabbit by repeated immunization with human IgG fragment.
Isotype IgG
Specificity IgG F(ab')2
Characteristics Anti-Human IgG F(ab')2 antibody detects human IgG F(ab')2 fragments. This secondary antibody anti-Human IgG F(ab')2 is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Human IgG F(ab')2 Antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.
Concentration Definition: by UV absorbance at 280 nm
Purification This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG, Human IgG F (ab')2 and Human Serum.
Sterility Sterile filtered
Background Synonyms: F(ab')2 Human IgG Antibody, Rb-a-Human F(ab')2 IgG, F(ab')2 Human IgG Antibody in Rabbit, Rabbit IgG Secondary Antibody.
Research Area Immunology, Secondary Antibodies
Application Notes Anti-Human IgG F(ab') 2 antibody is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Restrictions For Research Use only
Format Liquid
Concentration 2.2 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Background publications Titus, Haugland, Sharrow, Segal: "Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies." in: Journal of immunological methods, Vol. 50, Issue 2, pp. 193-204, 1982 (PubMed).