Chicken anti-Mouse IgG (Heavy & Light Chain) Antibody (HRP)
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- Target See all IgG products
- IgG
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Mouse
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Host
- Chicken
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Clonality
- Polyclonal
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Conjugate
- HRP
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Application
- ELISA
- Specificity
- IgG (H&L)
- Characteristics
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Anti-Mouse (H&L) peroxidase conjugated antibody generated in chicken detects specifically mouse IgG (H&L). This secondary peroxidase conjugated antibody anti-mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm - Immunogen
- Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in chicken.
- Isotype
- IgG
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- Application Notes
- Antibody Anti-Mouse IgG (H&L) peroxidase conjugated has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Preservative
- Gentamicin sulfate
- Handling Advice
- Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
- Storage
- 4 °C
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
- Synonyms: Mouse IgG (H&L) Antibody, Ck-a-Mouse IgG(H&L) Peroxidase conjugated, Mouse IgG(H&L) Peroxidase Antibody in chicken, Chicken IgG (H&L) peroxidase conjugated Secondary Antibody.
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