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Donkey anti-Mouse (Murine) IgG (Heavy & Light Chain) Antibody (Rhodamine)

Details for Product No. ABIN101711, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
4516
1866
1731
962
643
277
204
57
35
34
31
8
8
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Mouse (Murine)
2446
2329
2045
1530
1264
628
561
386
328
310
310
304
293
214
159
112
105
76
32
24
21
13
12
8
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
Host
Donkey
6065
4198
1506
573
545
298
85
49
43
8
8
4
4
3
1
1
Clonality
Polyclonal
Conjugate
Rhodamine
1916
1711
1552
1135
605
529
522
265
198
181
172
161
156
155
127
79
69
47
43
37
31
23
20
19
19
17
15
14
14
14
14
13
11
10
10
10
10
10
10
9
9
9
9
9
8
8
8
8
8
8
8
8
8
7
7
7
6
6
6
6
6
6
5
5
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
FLISA, Immunomicroscopy (IM), Western Blotting (WB)
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Immunogen Mouse IgG whole molecule
Isotype IgG
Specificity IgG (H&L)
Characteristics Concentration Definition: by UV absorbance at 280 nm

Label: Tetramethylrhodamine isothiocyanante (TRITC)
Molecular Weight 444 daltons.
Absorption Wavelength: 550 nm.
Emission Wavelength: 570 nm.
Fluorochrome/Protein Ratio: 3.0 moles TRITC per mole of Donkey IgG.
Purification This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Mouse IgG and Mouse Serum.
Research Area Immunology, Secondary Antibodies
Application Notes This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Handling Advice Product is photosensitive and should be protected from light.
Background publications Titus, Haugland, Sharrow, Segal: "Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies." in: Journal of immunological methods, Vol. 50, Issue 2, pp. 193-204, 1982