Donkey anti-Mouse (Murine) IgG (Heavy & Light Chain) Antibody (Rhodamine)

Details for Product No. ABIN101711, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
4762
2573
1887
1055
1007
843
304
273
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
1
Reactivity
Mouse (Murine)
2816
2785
2375
1734
1463
781
607
425
365
338
331
324
307
290
169
148
126
114
41
29
27
25
12
11
9
8
6
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
Host
Donkey
6775
4670
1875
826
703
396
88
54
52
21
9
8
5
5
2
1
1
Clonality
Polyclonal
Conjugate
Rhodamine
2206
1871
1720
1369
765
678
519
297
297
284
274
268
217
188
178
124
103
79
69
60
54
40
35
32
29
22
22
20
19
19
19
19
16
16
15
15
15
15
15
14
12
12
11
11
10
10
9
9
9
9
9
9
9
8
8
8
8
7
7
7
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
FLISA, Immunomicroscopy (IM), Western Blotting (WB)
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Immunogen Mouse IgG whole molecule
Isotype IgG
Specificity IgG (H&L)
Characteristics Concentration Definition: by UV absorbance at 280 nm

Label: Tetramethylrhodamine isothiocyanante (TRITC)
Molecular Weight 444 daltons.
Absorption Wavelength: 550 nm.
Emission Wavelength: 570 nm.
Fluorochrome/Protein Ratio: 3.0 moles TRITC per mole of Donkey IgG.
Purification This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Mouse IgG and Mouse Serum.
Research Area Immunology, Secondary Antibodies
Application Notes This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Handling Advice Product is photosensitive and should be protected from light.
Background publications Titus, Haugland, Sharrow, Segal: "Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies." in: Journal of immunological methods, Vol. 50, Issue 2, pp. 193-204, 1982 (PubMed).