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Donkey anti-Mouse (Murine) IgG (Heavy & Light Chain) Antibody (Rhodamine)

Details for Product No. ABIN101711, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
4370
1723
1662
896
804
618
560
280
35
34
31
8
8
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Mouse (Murine)
2657
2602
2294
1653
1387
733
606
416
348
336
332
325
306
259
165
148
118
112
41
27
21
21
12
11
7
5
5
5
4
4
4
4
4
4
4
4
4
3
3
2
2
1
1
1
1
Host
Donkey
6517
4559
1742
767
646
367
93
51
50
8
8
5
4
3
1
1
Clonality
Polyclonal
Conjugate
Rhodamine
2123
1813
1683
1296
720
632
514
301
283
282
272
264
212
186
178
117
81
79
69
54
49
40
35
25
23
21
19
18
15
15
15
15
14
14
14
14
13
11
10
10
10
10
10
10
9
9
9
9
9
8
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
FLISA, Immunomicroscopy (IM), Western Blotting (WB)
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Immunogen Mouse IgG whole molecule
Isotype IgG
Specificity IgG (H&L)
Characteristics Concentration Definition: by UV absorbance at 280 nm

Label: Tetramethylrhodamine isothiocyanante (TRITC)
Molecular Weight 444 daltons.
Absorption Wavelength: 550 nm.
Emission Wavelength: 570 nm.
Fluorochrome/Protein Ratio: 3.0 moles TRITC per mole of Donkey IgG.
Purification This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Mouse IgG and Mouse Serum.
Research Area Immunology, Secondary Antibodies
Application Notes This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Handling Advice Product is photosensitive and should be protected from light.
Background publications Titus, Haugland, Sharrow, Segal: "Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies." in: Journal of immunological methods, Vol. 50, Issue 2, pp. 193-204, 1982 (PubMed).