You are viewing an incomplete version of our website. Please click to reload the website as full version.

Donkey anti-Mouse (Murine) IgG (Heavy & Light Chain) Antibody (Rhodamine)

Details for Product No. ABIN101711, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
4382
2530
1757
1035
925
824
306
270
35
34
31
8
6
5
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
2644
2621
2280
1629
1374
735
603
422
361
333
329
321
308
258
165
147
124
112
41
27
25
21
12
11
9
8
6
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Host
Donkey
6519
4478
1761
774
636
365
87
51
50
9
8
5
4
3
1
1
Clonality
Polyclonal
Conjugate
Rhodamine
2103
1798
1649
1306
717
629
522
296
294
284
274
267
216
187
178
117
101
79
69
55
54
40
35
25
23
22
19
19
19
19
18
15
15
15
15
15
15
15
14
13
12
10
10
10
10
9
9
9
9
9
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
FLISA, Immunomicroscopy (IM), Western Blotting (WB)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Immunogen Mouse IgG whole molecule
Isotype IgG
Specificity IgG (H&L)
Characteristics Concentration Definition: by UV absorbance at 280 nm

Label: Tetramethylrhodamine isothiocyanante (TRITC)
Molecular Weight 444 daltons.
Absorption Wavelength: 550 nm.
Emission Wavelength: 570 nm.
Fluorochrome/Protein Ratio: 3.0 moles TRITC per mole of Donkey IgG.
Purification This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Mouse IgG and Mouse Serum.
Research Area Immunology, Secondary Antibodies
Application Notes This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Handling Advice Product is photosensitive and should be protected from light.
Background publications Titus, Haugland, Sharrow, Segal: "Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies." in: Journal of immunological methods, Vol. 50, Issue 2, pp. 193-204, 1982 (PubMed).