Goat anti-Mouse IgG (Heavy & Light Chain) Antibody (Texas Red (TR)) - Preadsorbed

Catalog No. ABIN101770

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: Texas Red (TR)

Application: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Specificity: IgG (H&L)

Characteristics: Mouse IgG (H&L) Antibody Texas Red™ Conjugated Pre-Adsorbed antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Mouse IgG whole molecule

Isotype: IgG

Labeling Ratio: 3.9

Application Details

Application Notes:

Application Note: Mouse IgG (H&L) Antibody Texas Red™ Conjugated Pre-Adsorbed antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Comment:

Texas Red™ is a registered trademark of Molecular Probes Inc.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Product is photosensitive and should be protected from light.

Storage: RT,4 °C,-20 °C

Expiry Date: 12 months

References

Chaillou, Zhang, McCarthy: "Expression of Muscle-Specific Ribosomal Protein L3-Like Impairs Myotube Growth." in: Journal of cellular physiology, Vol. 231, Issue 9, pp. 1894-902, (2017) (PubMed).

Fry, Lee, Mula, Kirby, Jackson, Liu, Yang, Mendias, Dupont-Versteegden, McCarthy, Peterson: "Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity without affecting sarcopenia." in: Nature medicine, Vol. 21, Issue 1, pp. 76-80, (2015) (PubMed).

Liu, Fry, Mula, Jackson, Lee, Peterson, Yang: "Automated fiber-type-specific cross-sectional area assessment and myonuclei counting in skeletal muscle." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 115, Issue 11, pp. 1714-24, (2014) (PubMed).

Fry, Lee, Jackson, Kirby, Stasko, Liu, Dupont-Versteegden, McCarthy, Peterson: "Regulation of the muscle fiber microenvironment by activated satellite cells during hypertrophy." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 28, Issue 4, pp. 1654-65, (2014) (PubMed).

Mula, Lee, Liu, Yang, Peterson: "Automated image analysis of skeletal muscle fiber cross-sectional area." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 114, Issue 1, pp. 148-55, (2013) (PubMed).

McCarthy, Mula, Miyazaki, Erfani, Garrison, Farooqui, Srikuea, Lawson, Grimes, Keller, Van Zant, Campbell, Esser, Dupont-Versteegden, Peterson: "Effective fiber hypertrophy in satellite cell-depleted skeletal muscle." in: Development (Cambridge, England), Vol. 138, Issue 17, pp. 3657-66, (2011) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Goat Anti Mouse IgG (H&L) Texas Red™ Conjugated Antibody Pre-Adsorbed, Goat Anti-Mouse IgG Antibody Texas Red™ Conjugation

Background: Anti-Mouse IgG Texas Red Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.