Rabbit anti-Pig IgG (F(ab')2 Region) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed
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- Target See all IgG products
- IgG
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Binding Specificity
- F(ab')2 Region
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Reactivity
- Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Alkaline Phosphatase (AP)
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- IgG F(ab')2
- Characteristics
- Concentration Definition: by UV absorbance at 280 nm
- Purification
- Preadsorption: Solid phase absorption
- Sterility
- Sterile filtered
- Immunogen
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Immunogen: Swine IgG F(ab')2 fragment
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Swine IgG F(ab')2 antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.
ELISA Dilution: 1:1,000 - 1:3,000
Western Blot Dilution: 1:500 - 1:2,000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - Storage
- 4 °C
- Expiry Date
- 12 months
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
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Synonyms: Rabbit Anti-Swine IgG F(ab')2 alkaline phosphatase Conjugated Antibody, Rabbit Anti-Swine IgG Fab2 alkaline phosphatase Conjugated Antibody, Rabbit Anti-Swine IgG Fab2 Fragment Antibody alk phos Conjugation
Background: Anti-Swine IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the Swine IgG molecule. Swine IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind Swine IgG F(c) will not bind Swine IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Swine IgG F(ab')2 is conjugated to alkaline phosphatase.
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