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Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody

Antigen

IgG

Binding Site
Alternatives

Heavy & Light Chain

Protein Type

Native

Clonality Polyclonal
Host
Reactivity
Alternatives

Rabbit

Conjugate
Alternatives Un-conjugated
Application
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunodiffusion (ID), Labeling (Lbl)
1 reference available
Catalog no. ABIN102001
Quantity 1 mg  (1.0 mg/mL)  (Variants)
Price 132.00 $   Plus shipping costs $45.00
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Additional Information

Immunogen Anti-Rabbit IgG was produced by repeated immunization with rabbit whole IgG molecule in goat.
Format Liquid
Reconstitution Restore with deionized water (or equivalent)
Isotype IgG
Description Anti-Rabbit IgG antibody generated in goat detects specifically rabbit IgG. This secondary antibody anti-Rabbit is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Characteristics Concentration Definition: by UV absorbance at 280 nm
Specificity IgG (H&L)

Application Details

Application Notes Anti-Rabbit IgG (H&L) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user
ELISA Dilution: 1:25,000 - 1:100,000
Immunohistochemistry Dilution: 1:500 - 1:2,000
Western Blot Dilution: 1:500 - 1:2,000
Concentration 1.0 mg/mL
Purification Sterile filtered
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative 0.01% (w/v) Sodium azide
Storage Expiration date is one (1) year from date of opening. None. Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. 
Research Area Immunology, Secondary Antibodies
Restrictions For Research Use only

Publications

Titus JA, Haugland R, Sharrow SO et al.: "Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies." in: J Immunol Methods, Vol. 50, Issue 2, pp. 193-204, 1982 (PubMed).

Alternatives

Alternatives for antigen "IgG", type "Secondary Antibodies"
Hosts Goat (3753), Rabbit (2828), Donkey (1028), Chicken (371), Mouse (290), Sheep (200), Guinea Pig (29), Rat (20), Pig (14), Duck (6), Horse (5), Cat (Feline) (2), Hamster (1), Human (1)
Reactivities Mouse (Murine) (1424), Human (1370), Rabbit (1140), Rat (Rattus) (946), Goat (711), Guinea Pig (389), Sheep (Ovine) (348), Cow (Bovine) (273), Cat (Feline) (226), Horse (Equine) (201), Dog (Canine) (197), Pig (Porcine) (186), Hamster (176), Chicken (172), Donkey (75), Monkey (74), Golden Syrian Hamster (69), Llama (51), Armenian Hamster (27), Mink (18), Syrian Hamster (15), Turkey (13), Ferret (12), Bird (Avian) (6), Rhesus Monkey (5), Deer (4), Duck (3), Pigeon (3), mouse (Murine) (1)
Conjugates HRP (1246), FITC (1160), Biotin (986), Alkaline Phosphatase (AP) (856), Texas Red (TR) (566), TRITC (376), Rhodamine (364), DyLight 594 (199), DyLight 800 (186), PE (143), DyLight 650 (116), DyLight 633 (115), DyLight 550 (113), DyLight 350 (96), RPE (64), Cy3 (42), Cy5 (39), Gold (37), APC (34), Alexa Fluor 647 (34), Alexa Fluor 350 (33), Alexa Fluor 488 (33), Alexa Fluor 555 (33), Cy5.5 (33), Cy7 (33), PE-Cy3 (33), PE-Cy5 (33), PE-Cy5.5 (33), PE-Cy7 (33), Agarose Beads (27), DyLight 488 (20), SureLight® R-PE (15), DyLight 405 (13), DyLight 680 (13), Atto 425 (10), Atto 488 (10), Atto 532 (10), Atto 550 (10), Atto 594 (10), Atto 647N (10), Atto 655 (10), Colloidal Gold (10nm) (8), Colloidal Gold (5nm) (7), SPRD (7), Beads (6), Peroxidase (POD) (6), Poly-HRP40 (4), beta-Gal (4), Poly-HRP (3), PromoFluor-350 (3), PromoFluor-405 (3), PromoFluor-415 (3), PromoFluor-488 Premium (3), PromoFluor-488LSS (3), PromoFluor-500LSS (3), PromoFluor-505 (3), PromoFluor-510LSS (3), PromoFluor-514LSS (3), PromoFluor-520LSS (3), PromoFluor-532 (3), PromoFluor-546 (3), PromoFluor-555 (3), PromoFluor-590 (3), PromoFluor-610 (3), PromoFluor-633 (3), PromoFluor-647 (3), PromoFluor-670 (3), PromoFluor-680 (3), PromoFluor-700 (3), PromoFluor-750 (3), PromoFluor-770 (3), PromoFluor-780 (3), PromoFluor-840 (3), AMCA (2), Chromeo™ 488 (2), Chromeo™ 546 (2), Colloidal Gold (20nm) (2), SureLight® P3 (2), Chromeo™ 494 (1), Chromeo™ 642 (1), Colloidal Gold (15nm) (1), DTAF (1), FL (1), PE,Cy7 (1), PE,RPE (1), Poly-HRP80 (1),  Texas Red (TR) (1)
Epitopes Heavy & Light Chain (3733), Fc Region (746), F(ab')2 Region (545), Fc Region, Chain gamma (78), Heavy Chain (57), Chain gamma (56), H&L (45), Fab Region (42), Chain gamma 1,Chain gamma 2b,Chain gamma 2a,Chain gamma 3 (14), Light Chain (7), Chain gamma,Fc Region (4), Fd Region (4), N-Term (2), Native Conformation (2), Heavy Chain, Fc Region (1)