Rabbit anti-Mouse (Murine) Immunoglobulin G2a (IgG2a) Antibody (Rhodamine)

Antigen: Immunoglobulin G2a (IgG2a)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Conjugate: Rhodamine

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM), Flow Cytometry (FACS)

Catalog no.: ABIN102382

Additional Information

Alternative name: IgG2a

Immunogen: Mouse IgG2a heavy chain

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Isotype: IgG

Description: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Specificity: IgG2a

Application Details

Application Notes: FLISA Dilution: 1:10,000 - 1:50,000
Fluorochrome Protein Value: 2.4
IF Microscopy Dilution: 1:1,000 - 1:5,000

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium azide

Storage: Expiration date is one (1) year from date of opening. 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free. Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. 

Research Area: Secondary Antibodies

Restrictions: For Research Use only

Publications

Titus JA, Haugland R, Sharrow SO et al.: "Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies." in: J Immunol Methods, Vol. 50, Issue 2, pp. 193-204, 1982 (PubMed).