Goat anti-Mouse IgG2a Antibody (TRITC) - Preadsorbed

Catalog No. ABIN376753

Quick Overview for Goat anti-Mouse IgG2a Antibody (TRITC) - Preadsorbed (ABIN376753)

Target: IgG2a

Clonality: Polyclonal

Conjugate: TRITC

Application: Immunocytochemistry (ICC), FLISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Goat

Purpose: Goat Anti-Mouse IgG2a, Human ads-TRITC

Specificity: Reacts with the heavy chain of mouse IgG 2a as demonstrated by ELISA and flow cytometry.

Characteristics: Goat Anti-Mouse IgG2a-TRITC antibody with minimal reactivity to human proteins for use in immunohistochemistry / immunocytochemistry assays.

Purification:

Purified by affinity chromatography on mouse IgG2a covalently linked to agarose, cross-adsorbed against mouse IgG1, IgG2b, IgG3, IgM, and IgA / human immunoglobulins and pooled sera for minimal reactivity

Preadsorption: Human pre-adsorbed

Immunogen: Mouse IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pooled antisera from goats hyperimmunized with mouse IgG2a

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline containing < 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C

References

Graver, Boots, Haacke, Diepstra, Brouwer, Sandovici: "Massive B-Cell Infiltration and Organization Into Artery Tertiary Lymphoid Organs in the Aorta of Large Vessel Giant Cell Arteritis." in: Frontiers in immunology, Vol. 10, pp. 83, (2019) (PubMed).

Baldwin, Singh, Codullo, King, Wilson, McInnes, Graham: "Elevated ACKR2 expression is a common feature of inflammatory arthropathies." in: Rheumatology (Oxford, England), Vol. 56, Issue 9, pp. 1607-1617, (2017) (PubMed).

Klingberg, Chow, Koehler, Boo, Buscemi, Quinn, Costell, Alman, Genot, Hinz: "Prestress in the extracellular matrix sensitizes latent TGF-β1 for activation." in: The Journal of cell biology, Vol. 207, Issue 2, pp. 283-97, (2015) (PubMed).

Target Details

Target: IgG2a

Target Type: Antibody

Background:

Description: Goat Anti-Mouse IgG2a, Human ads-TRITC

NCBI_Official_Symbol: Ighg2a

Gene ID: 380793

UniProt: P01863