IL1R2 antibody

Catalog No. ABIN103286

Supplier Product No.: 209-401-305

This Rabbit Polyclonal antibody specifically detects IL1R2 in WB, ELISA, FACS and IP. It exhibits reactivity toward Human.

Quick Overview for IL1R2 antibody (ABIN103286)

Target: IL1R2 (Interleukin 1 Receptor, Type II (IL1R2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL1R2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP)

Product Details anti-IL1R2 Antibody

Supplier Product No.: 209-401-305

Supplier: Rockland

Purpose: IL-1 Receptor Type II Antibody

Cross-Reactivity (Details): The antibody is directed against the extracellular domain of the human IL-1R type II, but precipitates the intact receptor expressed on human cells.

Characteristics: Synonyms: rabbit anti-IL-1 Receptor Type II Antibody, rabbit anti-interleukin-1 receptor beta type II antibody, IL1R Beta antibody, Il1r-2 antibody, IL1R2 antibody, IL1RB antibody, Interleukin 1 receptor beta type II antibody

Purification: This is an IgG preparation of whole rabbit serum purified by Protein A chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human IL-1R type II.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Application Note: This IgG fraction antibody of anti-Human IL-1 Receptor Type II (IL-1RII) has been tested for use in ELISA and Western Blot and is suitable for use in radioimmunoassays, flow cytometry and immunoprecipitation. Reactivity in other immunoassays is unknown.  IL-1RII has been termed p68 or CD121 in some laboratories.  IL-1RII has been shown to bind IL-1, IL-1a and IL-1b. This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L] (code # 611-1302) and TMB as a substrate.  A working dilution range of 1:200 to 1:400 is suggested for immunoblot. This product has been assayed by ELISA against soluble (extracellular) IL-1RII using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code # 611-1302) and ABTS as a substrate for 30 minutes at room temperature.  A working dilution range of 1:1,000 to 1:2000 is suggested for ELISA.  For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. A dilution range of 1:400 to 1:800 is suggested for immunoprecipitation.  For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background.  A dilution of 1:8,000 is suggested for radioimmunoassay. 

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for IL1R2

Target: IL1R2 (Interleukin 1 Receptor, Type II (IL1R2))

Alternative Name: IL1R2

Background: Background: Interleukin 1 receptor type II binds to IL-1 alpha, IL-1 beta and IL1R1/IL1RA. Anti-IL-1 Receptor Type II antibody is ideal for investigators involved in Immunology and Stem Cell research.

Gene ID: 7850

UniProt: P27930

Pathways: NF-kappaB Signaling