CD43 antibody

Catalog No. ABIN1042482

Cited in 3+ publications. The Mouse Monoclonal anti-CD43 antibody (Clone MT1) (ABIN1042482) specifically detects CD43 in WB, IHC (p) and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for CD43 antibody (ABIN1042482)

Target: CD43 (SPN) (Sialophorin (SPN))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD43 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MT1

Product Details anti-CD43 Antibody

Specificity: Clone MT1 produces mouse IgG1 immunoglobulins reactive with a 95-115 kD highly sialated glycoprotein. This antibody is used to identify B cell lines and myeloma cells. It may be used in the diagnosis of chronic lymphocytic leukemias (CLL), as an alternative to CD5. The antigen detected by MT1 is the only marker expressed on neoplasms of the very early precursor cells. In immunohistochemistry it reacts with T cells, macrophages, myeloid cells and B cells (weak). It is used for the typing of lymphomas in paraffin sections. CD43 may function as an adhesion molecule via interaction with CD54 although this has not been definitely established. It may also inhibit leucocyte interactions with other cells. The antigen is involved in the activation of T-cells, B cells, NK cells and monocytes. The membrane proximal portion of the cytoplasmic domain mediates an association with the cytoskeleton.

Characteristics: CD markers

Purification: Purified

Isotype: IgG1

Application Details

Application Notes: The antibody is suitable for flow cytometry for analysis of blood and bone marrow samples or in immunohistochemistry using cytospots, paraffin or frozen tissue sections. Optimal antibody dilution should be determined by titration.

Comment:

MT1 is a monoclonal antibody derived by fusion of X63 mouse myeloma cells with spleen cells from of Balb/c mice, intraperitoneally immunized with human monocytes isolated by centrifugal elutriation. Immunizations were carried out at least twice at 2-week intervals with: A) cell suspensions of a lymph node involved by Hodgkin's disease, B) cell suspensions of a lymph node involved by Bchronic lymphatic leukemia, C) cells of Hodgkin's cell line DEV, respectively. No adjuvants were used (3).

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.09% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-CD43 antibody (ABIN1042482)

Manjunath, Correa, Ardman, Ardman: "Negative regulation of T-cell adhesion and activation by CD43." in: Nature, Vol. 377, Issue 6549, pp. 535-8, (1995) (PubMed).

Schmid, Hediger, Brossmer, Collins, Haupt, Marti, Offner, Schaller, Takagaki, Walsh: "Amino acid sequence of human plasma galactoglycoprotein: identity with the extracellular region of CD43 (sialophorin)." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 2, pp. 663-7, (1992) (PubMed).

Poppema, Hollema, Visser, Vos: "Monoclonal antibodies (MT1, MT2, MB1, MB2, MB3) reactive with leukocyte subsets in paraffin-embedded tissue sections." in: The American journal of pathology, Vol. 127, Issue 3, pp. 418-29, (1987) (PubMed).

Target Details for CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Alternative Name: CD43

Background: CD43 antigen is expressed on all T cells, NK cells, myeloid cells and monocytes as well as on CD5 positive mature B cells. It is also present on all immature hematopoietic cells in the bone marrow. The antigen is deficient in patients with Wiskott-Aldrich syndrome. A soluble form of CD43 is present in human serum.

Pathways: Regulation of Leukocyte Mediated Immunity